Identification of hexahydro-1,3,5-trinitro-1,3,5-triazine-degrading microorganisms via 15N-stable isotope probing.
Identification of hexahydro-1,3,5-trinitro-1,3,5-triazine-degrading microorganisms via 15N-stable isotope probing.
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通过 15N 稳定同位素探测鉴定六氢-1,3,5-三硝基-1,3,5-三嗪降解微生物。
DOI：
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发表时间：
2009
影响因子：
11.4
通讯作者：
K. Chu
中科院分区：
文献类型：
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作者：
H. Roh;ChangJiang Yu;M. Fuller;K. Chu
本文报道了应用15 N稳定同位素探针技术（SIP）鉴定地下水微宇宙中利用黑索今（RDX）的活性微生物。15个16 S rRNA基因序列来自15 N-DNA部分（贡献于能够使用RDX作为氮源的活性微生物）的缩影接收奶酪乳清。16 S rRNA基因序列属于放线菌门（2个克隆）、α-变形菌门（7个克隆）和γ-变形菌门（6个克隆）。除了与两种已知的RDX降解菌（阴沟肠杆菌和荧光假单胞菌I-C）具有高度相似性的5个序列外，我们的研究结果表明，遗传多样性不同的微生物能够使用RDX作为氮源。从15 N-DNA部分检测到xplA基因（已知的RDX降解分解代谢基因）的六个序列。xplA基因序列与Rhodococcus sp.DN22（一种已知的RDX利用者）的xplA基因有96-99%的相似性，表明其它RDX利用者可能含有xplA样基因。从未富集的，RDX污染的地下水中回收的25个16 S rRNA基因序列与从奶酪乳清修正的微观世界的15 N-DNA部分中获得的序列不同。我们的研究结果表明，活跃的RDX利用者可以刺激营养源的添加，即使他们是在低密度，使用15 N-SIP可以identifythese功能的微生物群落的成员。
This study reported the application of 15N-stable isotope probing (SIP) to identify active hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)-utilizing microorganisms in groundwater microcosms. Fifteen 16S rRNA gene sequences were derived from the 15N-DNA fraction (contributed from active microorganisms capable of using RDX as a nitrogen source) of microcosms receiving cheese whey. The 16S rRNA gene sequences belonged to Actinobacteria (two clones), alpha-Proteobacteria (seven clones), and gamma-Proteobacteria (six clones). Except for five sequences with high similarity to two known RDX degraders (Enterobacter cloacae and Pseudomonas fluorescens I-C), our results suggested that phylogenetically diverse microorganisms were capable of using RDX as a nitrogen source. Six sequences of the xplA gene (a known RDX-degrading catabolic gene) were detected from the 15N-DNA fraction. The xplA gene sequences were 96-99% similar to the xplA gene of Rhodococcus sp. DN22(a known RDX utilizer), suggesting that other RDX utilizers might contain xplA-like genes. Twenty-five 16S rRNA gene sequences recovered from the unenriched, RDX-contaminated groundwater clustered differently from those obtained from the 15N-DNA fraction of the cheese-whey-amended microcosm. Our results suggested that active RDX utilizers can be stimulated by nutrient source additions even if they are present at low densities, and that use of 15N-SIP can identifythese functional members of the microbial community.