The Patrol Yeast: A new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning
The Patrol Yeast: A new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning
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巡逻酵母：一种配备抗体受体嵌合体的新型生物传感器，可检测一系列与食物中毒相关的有毒物质
DOI：
10.1016/j.bios.2022.114793
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发表时间：
2023
影响因子：
12.6
通讯作者：
Hiroshi Ueda
中科院分区：
文献类型：
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作者：
Jiulong Su;Bo Zhu;Akihito Inoue;Hiroyuki Oyama;Izumi Morita;Jinhua Dong;Takanobu Yasuda;Yoshiko Sugita-Konishi;Tetsuya Kitaguchi;Norihiro Kobayashi;Shiro Miyake;Hiroshi Ueda
面包酵母是一种有吸引力的宿主，具有既定的安全性和稳定性。已经开发了许多基于酵母的生物传感器，但是跨膜信号转导尚未用于利用抗原-抗体相互作用检测膜不可渗透物质。因此，我们创建了Patrol Yeast，这是一种新型的基于酵母的免疫传感器，用于检测各种目标，特别是食品中的有毒物质。基于膜的酵母双杂交系统，使用分裂泛素被成功地用于检测实际重要的浓度范围内的咖啡因和黄曲霉毒素使用分离的可变区的抗体。此外，使用特异性单链抗体检测肠毒素大肠杆菌O 157，其中添加了Zyrosin酶以部分破坏细胞壁。分泌型Cypridinaluciferase报告基因的掺入进一步简化了信号检测程序，而无需细胞裂解。该方法比使用哺乳动物细胞更具有成本效益和更快。检测各种目标的能力使Patrol Yeast成为确保食品和饮料安全以及解决其他环境和技术问题的宝贵工具。
Baker's yeast is an attractive host with established safety and stability characteristics. Many yeast-based biosensors have been developed, but transmembrane signal transduction has not been used to detect membrane-impermeable substances using antigen-antibody interactions. Therefore, we created Patrol Yeast, a novel yeast-based immunosensor of various targets, particularly toxic substances in food. A membrane-based yeast two-hybrid system using split-ubiquitin was successfully used to detect practically important concentration ranges of caffeine and aflatoxins using separated variable regions of an antibody. Moreover, enterohemorrhagicEscherichia coliO157 was detected using a specific single-chain antibody, in which Zymolyase was added to partially destroy the cell wall. The incorporation of secretedCypridinaluciferase reporter further simplified the signal detection procedures without cell lysis. The methodology is more cost-effective and faster than using mammalian cells. The ability to detect various targets renders Patrol Yeast a valuable tool for ensuring food and beverage safety and addressing other environmental and technological issues.