On the detection of paramagnetic species in the adriamycin-perfused rat heart: a reappraisal.
On the detection of paramagnetic species in the adriamycin-perfused rat heart: a reappraisal.
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关于阿霉素灌注大鼠心脏中顺磁性物质的检测：重新评估。
DOI：
10.1016/0006-291x(90)91428-u
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发表时间：
1990
影响因子：
3.1
通讯作者：
Baker,JE
中科院分区：
文献类型：
--
作者：
Kalyanaraman,B;Baker,JE
最近，L. Costaet 等人报道了在离体的、阿霉素灌注的大鼠心脏中直接检测到超氧阴离子和其他顺磁性物质[L. Costaet al.]。 Costaet al.(1988)生物化学。生物物理学。资源。通讯 153, 275–280]。我们重新评估了他们的研究结果，得出的结论是，从阿霉素灌注心脏获得的光谱的 ESR 参数与过氧自由基的参数一致，而不与超氧阴离子的参数一致。此外，过氧自由基的ESR谱很可能是由心肌组织研磨引起的伪影而产生的。这种伪影可能掩盖了阿霉素衍生的半醌自由基和心肌铁硫蛋白成分的 ESR 光谱。
Recently, L. Costaet al.reported the direct detection of the superoxide anion and other paramagnetic species in the isolated, adriamycin-perfused rat heart [L. Costaet al.(1988)Biochem. Biophys. Res. Commun.153, 275–280]. We have reevaluated the results of their study and concluded that the ESR parameters of the spectrum obtained from the adriamycin-perfused heart are consistent with that of the peroxyl radical and not with that of the superoxide anion. In addition, the ESR spectrum of the peroxyl radical is very likely produced as an artifact caused by the grinding of myocardial tissue. This artifact may mask the ESR spectra of the adriamycin-derived semiquinone radical and the iron-sulfur protein components of myocardium.