Bone formation and resorption of highly purified β-tricalcium phosphate in the rat femoral condyle
Bone formation and resorption of highly purified β-tricalcium phosphate in the rat femoral condyle
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DOI：
10.1016/j.biomaterials.2005.02.026
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发表时间：
2005-10-01
期刊：
影响因子：
14
通讯作者：
Endo, N
中科院分区：
文献类型：
--
作者：
Kondo, N;Ogose, A;Endo, N
本研究的目的是检查与高纯度β-磷酸三钙（β-TCP）植入大鼠股骨髁相关的时间组织学。分别于植入后第4、7、14、28和56天取材，用抗酒石酸酸性磷酸酶（TRAP）染色、免疫组化法检测吞噬细胞系统标志物EDI蛋白，并与地高辛标记的1型前胶原α 1链（COL 1A 1）、骨桥蛋白和骨钙素进行原位杂交。虽然在第4天没有观察到新骨，但β-TCP周围的成纤维细胞样细胞对COL 1A 1和骨桥蛋白mRNA呈阳性。第7天后出现新骨形成。在第7天OPN和EDI的双重染色中，β-TCP周围的大多数细胞对骨桥蛋白mRNA或ED 1蛋白呈阳性。但也有一些双阳性多核细胞，提示它们属于单核吞噬细胞系统。在第28天之后，用骨髓替换植入区域。多核TRAP阳性和ED 1阳性细胞在所有阶段都粘附于β-TCP，似乎是破骨细胞，它们持续吸收β-TCP。β-TCP具有良好的生物相容性，因为生物吸收和骨形成在植入后的早期阶段就开始了。(c)2005爱思唯尔有限公司保留所有权利。
The aim of this study was to examine the chronological histology associated with highly purified beta-tricalcium phosphate (beta-TCP) implanted in the rat Femoral condyle. Specimens were harvested on days 4, 7, 14, 28 and 56 after implantation, and were analyzed by tartrate-resistant acid phosphatase (TRAP) staining, immunohistochemistry of the EDI protein as a marker of the phagocyte system,and in situ hybridization with digoxigenin-labeled alpha 1 chain of type 1 procollagen (COL1A1),osteopontinand osteocalcin.beta-TCP was resorbed in a chronological manner. Although new bone was not observed on day 4, fibroblast-like cells around beta-TCP were positive for COL1A1 and osteopontin mRNA. New bone formation presented after day 7. In the double-staining for OPN and EDI on day 7, most cells around beta-TCP were positive for either osteopontin mRNA or ED1 protein. However, there were some doubly positive multinucleated cells, suggesting that they belonged to the mononuclear phagocyte system. After day 28, the implanted region was replaced with bone marrow. Multinucleated TRAP-positive and ED1-positive cells which adhered to beta-TCP at all stages seemed to be osteoclasts and they continuously resorbed beta-TCP. beta-TCP has a good biocompatibility since both bioresorption and bone formation started at an early stage after implantation. (c) 2005 Elsevier Ltd. All rights reserved.