Interactive effects of temperature and irradiance including spectral light quality on the photosynthesis of a brown alga Saccharina japonica (Laminariales) from Hokkaido, Japan
Interactive effects of temperature and irradiance including spectral light quality on the photosynthesis of a brown alga Saccharina japonica (Laminariales) from Hokkaido, Japan
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DOI：
10.1016/j.algal.2022.102777
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发表时间：
2022-07
期刊：
影响因子：
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通讯作者：
Aoi Shindo;I. A. Borlongan;Gregory N. Nishihara;Ryuta Terada
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作者：
Aoi Shindo;I. A. Borlongan;Gregory N. Nishihara;Ryuta Terada
利用脉冲幅度调制（PAM）叶绿素荧光仪和溶解氧传感器研究了温度和光照（包括光谱光质量）对产自日本北海道的海带光合作用的交互作用。在9个温度（4-36 °C，4个增量）下对3天培养过程中的最大量子产率（黑暗中）和有效量子产率（50 μmol光子m−2s−1）进行评估，结果表明温度阈值为24 °C，在此温度下最大量子产率早期下降，这可能是由于长时间黑暗中呼吸消耗了碳水化合物储备。与最大量子产率在4-20 °C之间相对稳定不同，50 μmol光子m−2s− 1下的有效量子产率在低温下逐渐下降，揭示了低温下的光敏感性。在4 ° C、16 ° C和24 °C下，在200（低）和1000（高）μmol光子m−2s− 1下慢性暴露6小时，在两种辐照度下，4 °C下的有效量子产率均显著下降;然而，只有在低辐照度下的处理在随后的12小时暗光处理后才完全恢复。此外，在16和24 °C的高辐照度下，弱光驯化后的处理的有效量子产率恢复到初始水平，表明在低温下加速的光抑制。在16 °C下，在蓝光（450 nm）、绿色（525 nm）、红光（660 nm）和白色光（金属卤化物灯）下，光合作用-辐照响应表明，最大光合产量出现在蓝光和白色光下。结果表明，在蓝光条件下，S. japonicapressed额外的证据，他们的生理适应冷温带潮下沃茨。
The interactive effects of temperature and irradiance including spectral light quality on the photosynthesis of a brown alga,Saccharina japonica(var.japonica, Laminariales) from Hokkaido, Japan were examined using a pulse amplitude modulation (PAM)-chlorophyll fluorometer and dissolved oxygen sensors. Assessment of maximum quantum yields (in darkness) and effective quantum yields (at 50 μmol photons m−2s−1) at nine temperatures (4–36 °C, four increments) during 3-d culture indicated a temperature threshold at 24 °C, with early decline of maximum quantum yields at such temperature, which could probably be due to exhaustion of carbohydrate reserves by respiration in prolonged darkness. Unlike maximum quantum yields that was relatively stable through 4–20 °C, effective quantum yields under 50 μmol photons m−2s−1gradually declined at low temperatures, revealing light sensitivity under chilling temperatures. Chronic 6-h exposures under 200 (low) and 1000 (high) μmol photons m−2s−1at 4, 16, and 24 °C generated a significant decline in effective quantum yields at 4 °C under both irradiances; however, only treatments under low irradiance fully recovered after a subsequent 12-h dim light treatment. Moreover, post-dim light acclimation effective quantum yields of treatments at 16 and 24 °C under high irradiance returned to initial levels, suggesting accelerated photoinhibition at low temperature. Photosynthesis–irradiance responses at 16 °C under blue (450 nm), green (525 nm), red (660 nm), and white light (metal halide lamp) showed that the maximum photosynthetic output occurred under blue and white lights. The photoinhibitory sensitivity to chilling-light stress and the enhanced photosynthesis under blue light ofS. japonicapresented additional evidence of their physiological adaptation to cold-temperate subtidal waters.