Phyllobacterium sophorae sp nova, a symbiotic, bacterium isolated from root nodules of Sophora flavescens
Phyllobacterium sophorae sp nova, a symbiotic, bacterium isolated from root nodules of Sophora flavescens
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槐豆新芽杆菌，一种从苦参根瘤中分离出来的共生细菌
DOI：
10.1099/ijs.0.067017-0
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发表时间：
2015-02-01
影响因子：
2.8
通讯作者：
Chen, Wen Feng
中科院分区：
文献类型：
--
作者：
Jiao, Yin Shan;Yan, Hui;Chen, Wen Feng
从苦参（Sophora flavescens）根瘤中分离到2株革兰氏阴性菌（CCBAU 03422（T）和CCBAU 03415），通过16 S rRNA和atpD基因的比较分析，将其鉴定为叶杆菌属（Phyllobacterium）。它们显示出与芸苔叶杆菌LMG 22836（T）99.8%的rRNA基因序列相似性，并且菌株CCBAU 03422（T）分别显示出与内生叶杆菌LMG 26470（T）和芸苔叶杆菌LMG 22836（T）91.2%和88.6%的atpD基因序列相似性。菌株CCBAU 03422（T）含有Q-10作为其主要醌，并显示出与相关种的模式菌株不同的细胞脂肪酸谱、碳源利用和其他表型特征。DNA-DNA相关性（低于48.8%）进一步证实了新菌株与相关种的模式菌株之间的差异。菌株CCBAU 03422（T）能有效地在其寄主植物苦参上固氮。根据上述结果，提出了一个新的种，命名为槐叶杆菌（Phyllobacterium soppellum），其模式菌株为CCBAU 03422（T）（=A-6-3（T）=LMG 27899（T）=HAMBI 3508（T））。
Two novel Gram-stain-negative strains (CCBAU 03422(T) and CCBAU 03415) isolated from root nodules of Sophora flavescens were classified phylogenetically into the genus Phyllobacterium based on the comparative analysis of 16S rRNA and atpD genes. They showed 99.8 % rRNA gene sequence similarities to Phyllobacterium brassicacearum LMG 22836(T), and strain CCBAU 03422(T) showed 91.2 and 88.6% atpD gene sequence similarities to strains Phyllobacterium endophyticum LMG 26470(T) and Phyllobacterium brassicacearum LMG 22836(T), respectively. Strain CCBAU 03422(T) contained Q-10 as its major quinone and showed a cellular fatty acid profile, carbon source utilization and other phenotypic characteristics differing from type strains of related species. DNA-DNA relatedness (lower than 48.8%) further confirmed the differences between the novel strains and the type strains of related species. Strain CCBAU 03422(T) could nodulate and fix nitrogen effectively on its original host plant, Sophora flavescens. Based upon the results mentioned above, a novel species named Phyllobacterium sophorae is proposed and the type strain is CCBAU 03422(T) (=A-6-3(T)=LMG 27899(T)=HAMBI 3508(T)).