Expression of 8-oxoguanine DNA glycosylase (OGG1) in Parkinson's disease and related neurodegenerative disorders
Expression of 8-oxoguanine DNA glycosylase (OGG1) in Parkinson's disease and related neurodegenerative disorders
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DOI：
10.1007/s00401-004-0937-9
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发表时间：
2005-04-01
影响因子：
12.7
通讯作者：
Hattori, N
中科院分区：
文献类型：
--
作者：
Fukae, J;Takanashi, M;Hattori, N
包括DNA氧化在内的氧化应激与帕金森病（PD）有关。我们假设DNA修复酶如8-氧鸟苷DNA糖基化酶（OGG1）参与PD过程。我们对PD患者和进行性核上性麻痹（PSP）和皮质基底变性（CBD）患者的大脑进行了免疫组织化学和生化研究，分别作为疾病对照和对照组。我们发现帕金森病患者黑质（SN）中OGG1酶的线粒体同工型表达水平较高。此外，Western blot分析显示，PD患者SN中OGG1水平较高。我们的结果表明氧化应激在PD发病过程中易感病变的重要性。
Oxidative stress including DNA oxidation is implicated in Parkinson's disease (PD). We postulated that DNA repair enzymes such as 8-oxoguanosine DNA glycosylase (OGG1) are involved in the PD process. We performed immunohistochemical and biochemical studies on brains of patients with PD and those of patients with progressive supranuclear palsy (PSP) and corticobasal degeneration (CBD) as disease controls, and control subjects. We found higher expression levels of mitochondrial isoforms of OGG1 enzymes in the substantia nigra (SN) in cases of PD. Furthermore, Western blot analysis revealed high OGG1 levels in the SN of the patients with PD. Our results indicate the importance of oxidative stress within the susceptible lesions in the pathogenesis of PD.