Seroprevalence of IgA and IgM antibodies to Bordetella pertussis in healthy Japanese donors: Assessment for the serological diagnosis of pertussis
Seroprevalence of IgA and IgM antibodies to Bordetella pertussis in healthy Japanese donors: Assessment for the serological diagnosis of pertussis
复制标题
DOI：
10.1371/journal.pone.0219255
复制
发表时间：
2019-07-01
期刊：
影响因子：
3.7
通讯作者：
Shibayama, Keigo
中科院分区：
文献类型：
--
作者：
Fumimoto, Rei;Otsuka, Nao;Shibayama, Keigo
百日咳是一种由革兰氏阴性菌百日咳杆菌引起的人类呼吸道感染。为了评估百日咳负担和疫苗效力，诊断和流行病学监测应基于准确和有效的诊断方法。最近，Novagnost百日咳杆菌伊加和IgM血清学诊断测试在日本被批准用于百日咳诊断。虽然抗百日咳毒素（PT）IgG检测已在世界范围内用于百日咳诊断，但对抗B。百日咳伊加和IgM测定。本研究检测了460例健康献血员血清中抗B抗体的阳性率。日本人群中百日咳伊加和IgM抗体水平及其与献血者年龄的相关性。我们的数据表明，抗B。百日咳伊加、IgM与年龄呈正相关（r = 0.27），与年龄呈负相关（r = -0.37; P < 0.001）。抗-B特异性分析显示抗-B的高滴度。成人（46-50岁）百日咳IgA抗体伊加;学龄儿童（6-10岁、11-15岁）百日咳IgM滴度高。当应用这些年龄的任意临界值时，17.6%和39.5%的健康供体被解释为百日咳阳性或抗B抗体不确定。百日咳伊加（46-50岁）和IgM（11-15岁）滴度。总体而言，我们的研究结果表明，Novagnost百日咳博德特氏菌伊加和IgM检测可能会受到受试者年龄的很大影响，从而限制了其对百日咳诊断的价值。
Pertussis is a human respiratory infection caused by the gram-negative bacterium, Bordetella pertussis. To evaluate the pertussis burden and vaccine efficacy, diagnosis and epidemiological surveillance should be based on accurate and valid diagnostic methods. Recently, the serodiagnostic tests Novagnost Bordetella pertussis IgA and IgM were approved in Japan for pertussis diagnostics. Although the anti-pertussis toxin (PT) IgG assay has been used for pertussis diagnosis worldwide, little is known about the anti-B. pertussis IgA and IgM assays. In this study, serum samples from 460 healthy donors were examined to determine the seroprevalence of anti-B. pertussis IgA and IgM in a Japanese population, and its correlation with donor age. Our data demonstrated that anti-B. pertussis IgA and IgM are positively and negatively correlated with age (r = 0.27, r = -0.37; P < 0.001, respectively). Age-specific analysis revealed high titers of anti-B. pertussis IgA in adults (46-50 years), while anti-B. pertussis IgM titers were high in schoolchildren (6-10, 11-15 years). When applying the arbitrary cut-off values for these ages, 17.6% and 39.5% of healthy donors were interpreted as pertussis-positive or indeterminate with anti-B. pertussis IgA (46-50 years) and IgM (11-15 years) titers, respectively. Overall, our findings indicated that the Novagnost Bordetella pertussis IgA and IgM testing could be greatly affected by subject age, limiting its value for pertussis diagnosis.