CDNA SEQUENCE AND DEDUCED AMINO-ACID-SEQUENCE OF THE PRECURSOR OF THE 37-KDA INNER ENVELOPE MEMBRANE POLYPEPTIDE FROM SPINACH-CHLOROPLASTS - ITS TRANSIT PEPTIDE CONTAINS AN AMPHIPHILIC ALPHA-HELIX AS THE ONLY DETECTABLE STRUCTURAL ELEMENT
CDNA SEQUENCE AND DEDUCED AMINO-ACID-SEQUENCE OF THE PRECURSOR OF THE 37-KDA INNER ENVELOPE MEMBRANE POLYPEPTIDE FROM SPINACH-CHLOROPLASTS - ITS TRANSIT PEPTIDE CONTAINS AN AMPHIPHILIC ALPHA-HELIX AS THE ONLY DETECTABLE STRUCTURAL ELEMENT
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DOI：
10.1111/j.1432-1033.1991.tb15714.x
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发表时间：
1991-01-30
期刊：
影响因子：
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通讯作者：
FLUGGE, UI
中科院分区：
文献类型：
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作者：
DRESESWERRINGLOER, U;FISCHER, K;FLUGGE, UI
我们提出了编码菠菜叶绿体37-kDa内包膜蛋白整个前体的cDNA克隆的核苷酸序列和推断的氨基酸序列。前体蛋白由344个氨基酸（M(r) 38976）组成。对体外转录和翻译的前体蛋白进行体外处理和放射序列分析，发现其传递肽仅由21个氨基酸残基组成。传递肽具有形成具有强疏水力矩的两亲性α -螺旋的潜力。据推测，该结构元素代表了一个祖先包膜靶向域。体外合成的前体蛋白被引导到叶绿体中，并以atp依赖的方式插入到包膜中。成熟蛋白（323个氨基酸残基，M(r) 36830）具有中等疏水性，仅包含一个位于c端的跨膜片段，可能将蛋白质固定在包膜内。
We present the nucleotide sequence and the deduced amino acid sequence of a cDNA clone that encodes the entire precursor of the 37-kDa inner envelope membrane protein from spinach chloroplasts. The precursor protein consists of 344 amino acids (M(r) 38976). In vitro processing followed by radiosequence analysis of the in vitro transcribed and translated precursor protein revealed that its transit peptide consists of only 21 amino acid residues. The transit peptide has the potential to form an amphiphilic alpha-helix with a strong hydrophobic moment. It is speculated that this structural element represents an ancestral envelope-targeting domain. The in vitro synthesized precursor protein is directed to the chloroplasts and it is inserted in to the envelope membrane in an ATP-dependent manner. The mature protein (323 amino acid residues, M(r) 36830) has a moderate hydrophobicity and contains only one membrane-spanning segment which is located at the C-terminus and possibly anchors the protein within the envelope membrane.