Identification of Hepatitis C Virus (HCV) Subtype 1b Strains That Are Highly, or Only Weakly, Associated with Hepatocellular Carcinoma on the Basis of the Secondary Structure of an Amino-Terminal Portion of the HCV NS3 Protein
Identification of Hepatitis C Virus (HCV) Subtype 1b Strains That Are Highly, or Only Weakly, Associated with Hepatocellular Carcinoma on the Basis of the Secondary Structure of an Amino-Terminal Portion of the HCV NS3 Protein
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基于 HCV NS3 蛋白氨基末端部分的二级结构鉴定与肝细胞癌高度相关或仅弱相关的丙型肝炎病毒 (HCV) 亚型 1b 菌株
DOI：
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发表时间：
2003
影响因子：
9.4
通讯作者：
H. Hotta
中科院分区：
文献类型：
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作者：
S. Ogata;Ruth Huab Florese;M. Nagano‐Fujii;R. Hidajat;Lin Deng;Y. Ku;Seitetsu Yoon;Takafumi Saito;S. Kawata;H. Hotta
本文分析了89例肝细胞癌（HCC）和78例非HCC患者的丙型肝炎病毒1b亚型（HCV-1b）分离株的NS 3蛋白。根据NS 3氨基端120个残基的二级结构，HCV-1 B分离株分为A组、B组和一个不确定组，每个不确定组又分为许多亚组，如A1-1、A1-2、A2-1、A2-2、B1-1、B1-2、B2-1、B2-2、C-1、C-2和C-3。在89例HCC患者中有53例（59.6%）和78例非HCC患者中有19例（24.4%）检出B1-1亚群HCV-1b分离株，两组间差异有统计学意义（P < 0.00001）。虽然分离株的数量很少，但亚组B2-1与HCC也高度相关，该亚组中的所有5株分离株均在HCC患者中发现（P < 0.05）。另一方面，A1-1亚群的HCV-1b分离株与HCC仅弱相关;它们在89例HCC患者中的6例（6.7%）和78例非HCC患者中的25例（32.1%）中被发现，两个患者组之间的差异具有统计学意义（P < 0.0001）。其他亚组，如A1-2、A2-1、B1-2、C-1、C-2和C-3，与HCC中度相关;它们在HCC患者中的分布模式与非HCC患者中的分布模式无显著差异。综上所述，我们的研究结果表明，HCV-1b分离的亚组B1-1和B2-1与肝癌高度相关，这种二级结构分析可能有助于预测发展肝癌的相对风险。
ABSTRACT The NS3 protein of hepatitis C virus subtype 1b (HCV-1b) isolates obtained from 89 patients with hepatocellular carcinoma (HCC) and 78 patients without HCC were analyzed. On the basis of the secondary structure of the amino-terminal 120 residues of NS3, HCV-1b isolates were classified into group A, group B, and an indeterminate group, each of which was further divided into a number of subgroups, such as A1-1, A1-2, A2-1, A2-2, B1-1, B1-2, B2-1, B2-2, C-1, C-2, and C-3. HCV-1b isolates of subgroup B1-1 were found in 53 (59.6%) of 89 patients with HCC and 19 (24.4%) of 78 patients without HCC, with the difference between the two patient groups being statistically significant (P < 0.00001). Although the number of isolates was small, subgroup B2-1 was also highly associated with HCC, with all five isolates in that subgroup being found in patients with HCC (P < 0.05). On the other hand, HCV-1b isolates of subgroup A1-1 were associated only weakly with HCC; they were found in 6 (6.7%) of 89 patients with HCC and in 25 (32.1%) of 78 patients without HCC, with the difference between the two patient groups being statistically significant (P < 0.0001). The other subgroups, such as A1-2, A2-1, B1-2, C-1, C-2, and C-3, were moderately associated with HCC; their distribution patterns among patients with HCC did not differ significantly from those among patients without HCC. Taken together, our results suggest that HCV-1b isolates of subgroups B1-1 and B2-1 are highly associated with HCC and that this secondary structure analysis may be useful for predicting the relative risk of developing HCC.