Identification of biochemical association of phycobilisome with photosystems in cyanobacterial state transition
Identification of biochemical association of phycobilisome with photosystems in cyanobacterial state transition
复制标题
蓝藻状态转变中藻胆体与光系统的生化关联的鉴定
DOI：
10.1093/abbs/gmu072
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Ma W
中科院分区：
文献类型：
--
作者：
Zhao J;Chen L;Gao F;Wang Q;Qiu Z;Ma W
状态转换是光系统II（PSII）和PSI之间能量分配的一种短期平衡机制。虽然光诱导蓝藻的状态转换已被认为是完全依赖于藻胆体（PBS）之间的PSII和PSI的运动，生化证据还没有得到明确的显示。在这项研究中，当细胞分别通过暴露于蓝色或绿色光而达到状态1或2时，我们使用甘氨酸甜菜碱锁定PBS与PSII或PSI的关联。随后，PBS反应中心通过蓝色非变性聚丙烯酰胺凝胶电泳和双向电泳解析，然后通过蛋白质印迹分析鉴定。结果表明，在野生型（WT）集胞藻中。菌株PCC 6803中，PBS核心在光诱导的状态1-状态2转变期间总是与PSII二聚体共迁移，但其杆在状态2中离开PSII二聚体，而不管其在状态1中的共迁移。在光诱导的状态2中，在WT中观察到PBS棒与PSI三聚体的共迁移，但在ΔndhB（M55）中没有观察到，Δ ndhB（M55）是一种状态2转换缺陷型突变体。本研究首次提供了蓝细菌状态转换过程中PBS与光系统相关的生化证据。
State transition is a short-term balance mechanism of energy distribution between photosystem II (PSII) and PSI. Although light-induced state transition in cyanobacteria has been suggested to depend completely on the phycobilisome (PBS) movement between PSII and PSI, the biochemical evidence has not been clearly shown. In this study, we locked the association of PBS with PSII or PSI using glycinebetaine when cells attain State 1 or 2 by exposure to light of blue or green, respectively. Subsequently, the PBS-reaction centers were resolved by blue native polyacrylamide gel electrophoresis and two-dimensional electrophoresis, and then identified by western blot analysis. The results showed that in wild-type (WT)Synechocystissp. strain PCC 6803, the PBS core always co-migrates with the PSII dimer during light-induced State 1–State 2 transition, but its rod leaves the PSII dimer in State 2 regardless of its co-migration in State 1. In the light-induced State 2, the co-migration of PBS rod with PSI trimer was observed in WT, but not in ΔndhB(M55), a State-2-transition-deficient mutant. This study first provided the biochemical evidence for the association of PBS with photosystems during cyanobacterial state transition.