THE N-END RULE IN BACTERIA
THE N-END RULE IN BACTERIA
复制标题
DOI：
10.1126/science.1962196
复制
发表时间：
1991-11-29
期刊：
影响因子：
56.9
通讯作者：
VARSHAVSKY, A
中科院分区：
文献类型：
--
作者：
TOBIAS, JW;SHRADER, TE;VARSHAVSKY, A
N-末端规则将蛋白质的体内半衰期与其氨基末端残基的身份联系起来。不同版本的N-末端规则在所有的真核生物检查。结果表明，大肠杆菌也具有N-末端规则途径。氨基末端的精氨酸、赖氨酸、亮氨酸、苯丙氨酸、酪氨酸和色氨酸赋予测试蛋白2分钟的半衰期;其他氨基末端残基赋予相同蛋白10小时以上的半衰期。氨基末端精氨酸和赖氨酸是大肠杆菌的二级去稳定残基。因为它们的活性取决于它们通过亮氨酸、苯丙氨酸转移RNA-蛋白转移酶与初级去稳定残基亮氨酸或苯丙氨酸的缀合。三磷酸腺苷依赖性蛋白酶Clp（Ti）是E.杆菌
The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Distinct versions of the N-end rule operate in all eukaryotes examined. It is shown that the bacterium Escherichia coli also has the N-end rule pathway. Amino-terminal arginine, lysine, leucine, phenylalanine, tyrosine, and tryptophan confer 2-minute half-lives on a test protein; the other amino-terminal residues confer greater than 10-hour half-fives on the same protein. Amino-terminal arginine and lysine are secondary destabilizing residues in E. coli because their activity depends on their conjugation to the primary destabilizing residues leucine or phenylalanine by leucine, phenylalanine-transfer RNA-protein transferase. The adenosine triphosphate-dependent protease Clp (Ti) is required for the degradation of N-end rule substrates in E. coli.