Cytochrome P450 monooxygenase-mediated permethrin resistance confers limited and larval specific cross-resistance in the southern house mosquito, Culex pipiens quinquefasciatus
Cytochrome P450 monooxygenase-mediated permethrin resistance confers limited and larval specific cross-resistance in the southern house mosquito, Culex pipiens quinquefasciatus
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DOI：
10.1016/j.pestbp.2007.06.006
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发表时间：
2007-11-01
影响因子：
4.7
通讯作者：
Scott, Jeffrev G.
中科院分区：
文献类型：
--
作者：
Hardstone, Melissa C.;Leichter, Cheryl;Scott, Jeffrev G.
细胞色素p450依赖的单加氧酶（p450）是一个重要的酶系统，可以代谢外源性化合物（如农药）和内源性化合物（如激素）。p450介导的代谢可导致杀虫剂（如拟除虫菊酯）的解毒，或可参与杀虫剂（如有机磷）的生物活化和解毒。采用标准回交方法从MAL株中分离出一株抗氯菊酯的致倦库蚊（ISOP450），对氯菊酯具有1300倍的抗性。ISOP450与敏感实验室菌株（SLAB）高度相关，对氯菊酯的高抗性完全是由于p450介导的解毒作用。这是首次在蚊子中分离和研究P450单加氧酶参与拟除虫菊酯类杀虫剂抗性而不受kdr混杂效应的影响。ISOP450的耐药是不完全显性的（D = +0.3），常染色体连锁，单因素遗传。它在幼虫中表达，但在成虫中不表达。对缺乏3-苯氧苄基部分的拟除虫菊酯（氰菊酯、氟氟菊酯、生物乙酰氰菊酯和联苯菊酯）的交叉抗性为1.5至12倍。ISOP450对含有-氰基的3-苯氧苄基拟除虫菊酯（分别为氯氰菊酯和溴氰菊酯）只有有限的交叉抗性（分别为6.6倍和11倍）。ISOP450对有机磷杀虫剂的交叉抗性检测显示，对杀虫硫磷的抗性为8倍，而对马拉硫磷（RR = 0.84）、双硫磷（RR = 0.73）和甲基对硫磷（RR = 0.55）的交叉抗性水平较低，但具有显著性。讨论了P450机制在杀虫剂抗性中的重要性和独特性。(C) 2007爱思唯尔公司版权所有。
The cytochrome P450-dependent monooxygenases (P450s) are an important enzymatic system that metabolizes xenobiotics (e.g., pesticides), as well as endogenous compounds (e.g., hormones). P450-mediated metabolism can result in detoxification of insecticides such as pyrethroids, or can be involved in the bioactivation and detoxification of insecticides such as organophosphates. We isolated (from the MAL strain) a permethrin resistant strain (ISOP450) of Culex pipiens quinquefasciatus, having 1300-fold permethrin resistance using standard backcrossing procedures. ISOP450 is highly related to the susceptible lab strain (SLAB) and the high resistance to permethrin is due solely to P450-mediated detoxification. This is the first time in mosquitoes that P450 monooxygenase involvement in pyrethroid resistance has been isolated and studied without the confounding effects of kdr. Resistance in ISOP450 is incompletely dominant (D = +0.3), autosomally linked, and monofactorally inherited. It is expressed in the larvae, but not in adults. Cross-resistance to pyrethroids lacking a 3-phenoxybenzyl moiety (tetramethrin, fenfluthrin, bioallethrin, and bifenthrin) ranged from 1.5- to 12-fold. ISOP450 had only limited (6.6- and 11-fold) cross-resistance to 3-phenoxybenzyl pyrethroids with an alpha-cyano group (cypermethrin and deltamethrin, respectively). Examination of cross-resistance patterns to organophosphate insecticides in ISOP450 showed an 8-fold resistance to fenitrothion, while low, but significant, levels of negative cross-resistance were found for malathion (RR = 0.84), temephos (RR = 0.73), and methyl-parathion (RR = 0.55). The importance and uniqueness of this P450 mechanism in insecticide resistance is discussed. (C) 2007 Elsevier Inc. All rights reserved.