Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics.
Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics.
复制标题
脱氢表雄酮对哮喘患者外周血单核细胞 Th2 细胞因子产生的影响。
DOI：
10.3904/kjim.2008.23.4.176
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发表时间：
2008-12
期刊：
影响因子：
--
通讯作者：
Won YH
中科院分区：
文献类型：
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作者：
Choi IS;Cui Y;Koh YA;Lee HC;Cho YB;Won YH
雄激素脱氢表雄酮 (DHEA) 通过下调小鼠的 Th2 反应来减轻过敏性炎症气道反应。本研究的目的是调查 DHEA 是否抑制哮喘患者培养的外周血单核细胞 (PBMC) 中 Th2 细胞因子的产生。连续六十一名疑似哮喘或非哮喘男性接受了哮喘检查。每个受试者的 PBMC 在有或没有 DHEA (0.01~10 µM) 的情况下培养 48 小时。通过酶联免疫吸附测定法测量培养物上清液中干扰素（IFN）-γ、白细胞介素（IL）-5和IL-10的浓度。在表现出乙酰甲胆碱气道高反应性 (AHR) 的受试者的 PBMC 中，DHEA 以剂量依赖性方式显着抑制 IL-10、IL-5 和 IFN-γ 的产生（全部 p<0.001），并倾向于增加 IFN-γ/IL-5 比率（p=0.087）。与没有 AHR 的受试者相比，DHEA (10 µM) 更大程度地抑制 AHR 受试者的细胞因子产生（IL-5：24.0±7.8% vs. 40.9±3.6%，p<0.01；IFN-γ：29.7±7.0% vs. 54.5±5.1%，p<0.01）。细胞因子抑制与 AHR、血清总 IgE 水平和皮肤对屋尘螨的反应性显着相关。 DHEA 同时抑制 Th1 和 Th2 反应，且存在 Th1 偏向性，抑制程度与 AHR 或特应性的严重程度相关。因此，DHEA 可能是治疗哮喘的有效疗法。
The androgen dehydroepiandrosterone (DHEA) attenuates allergic inflammatory airway reactions by down-regulating the Th2 response in mice. The purpose of this study was to investigate whether DHEA suppresses Th2 cytokine production in cultured peripheral blood mononuclear cells (PBMCs) from asthmatic patients. Sixty-one consecutive suspected asthmatic or non-asthmatic men underwent tests for asthma. PBMCs from each subject were cultured with and without DHEA (0.01~10 µM) for 48 h. The concentrations of interferon (IFN)-γ, interleukin (IL)-5, and IL-10 in the culture supernatant were measured via an enzyme-linked immunosorbent assay. In PBMCs from subjects exhibiting methacholine airway hyperresponsiveness (AHR), DHEA significantly suppressed IL-10, IL-5, and IFN-γ production in a dose-dependent manner (all p<0.001) and tended to increase the IFN-γ/IL-5 ratio (p=0.087). DHEA (10 µM) suppressed cytokine production to a greater degree in subjects with AHR compared with those without AHR (IL-5: 24.0±7.8% vs. 40.9±3.6%, p<0.01; IFN-γ: 29.7±7.0% vs. 54.5±5.1%, p<0.01). Cytokine suppression was significantly related to AHR, serum total IgE levels, and skin reactivity to house dust mites. DHEA suppressed both Th1 and Th2 responses, with a Th1 bias, and the degree of suppression was associated with the severity of AHR or atopy. Therefore, DHEA may be a useful therapy for asthma.