Molecular components of the mitotic spindle.
Molecular components of the mitotic spindle.
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有丝分裂纺锤体的分子成分。
DOI：
10.1002/bies.950140203
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Nislow,C
中科院分区：
文献类型：
--
作者：
Kuriyama,R;Nislow,C
有丝分裂纺锤体构成了细胞分裂过程中负责将遗传物质均匀分配到每个子细胞中的机制。它们是短暂的，因此结构非常不稳定，即使在执行其功能时也会改变其形态。有丝分裂细胞的生化、免疫学和遗传分析使我们能够识别在有丝分裂开始时被募集形成纺锤体的各种分子。评估这些分子在纺锤体微管的形成和动力学中的作用对于理解有丝分裂的分子基础及其调节非常重要。我们最近使用针对从培养的哺乳动物细胞中分离出的有丝分裂纺锤体产生的单克隆抗体探针，鉴定了一种新型有丝分裂特异性微管相关蛋白（MAP）。这种 95/105 kDa 抗原代表了纺锤体的独特成分，与迄今为止报道的任何其他 MAP 不同。抗体显微注射以阶段特异性和剂量依赖性方式导致有丝分裂抑制，表明该蛋白质是纺锤体的重要组成部分。
Mitotic spindles constitute the machinery responsible for equidistribution of the genetic material into each daughter cell during cell division. They are transient and hence quite labile structures, changing their morphology even while performing their function. Biochemical, immunological and genetic analyses of mitotic cells have allowed us to identify a variety of molecules that are recruited to form the spindle at the onset of mitosis. Evaluation of the roles of these molecules in both the formation and in the dynamics of spindle microtubules should be important for understanding the molecular basis of mitosis and its regulation. We have recently identified a novel mitosis‐specific microtubule‐associated protein (MAP) using a monoclonal antibody probe raised against the mitotic spindles isolated from cultured mammalian cells. This 95/105 kDa antigen represents a unique component of the spindle distinct from any of the other MAPs reported so far. Antibody microinjection resulted in mitotic inhibition in a stage‐specific and dosedependent manner, indicating that the protein is an essential spindle component.