Intrinsic optical signal imaging of glucose-stimulated insulin secreting β-cells.
Intrinsic optical signal imaging of glucose-stimulated insulin secreting β-cells.
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DOI：
10.1364/oe.19.000099
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发表时间：
2011-01-03
期刊：
影响因子：
3.8
通讯作者：
Yao XC
中科院分区：
文献类型：
--
作者：
Li YC;Cui WX;Wang XJ;Amthor F;Lu RW;Thompson A;Yao XC
Simultaneous monitoring of many functioning β-cells is essential for understanding β-cell dysfunction as an early event in the progression to diabetes. Intrinsic optical signal (IOS) imaging has been shown to allow high resolution detection of stimulus-evoked physiological responses in the retina and other neural tissues. In this paper, we demonstrate the feasibility of using IOS imaging for functional examination of insulin secreting INS-1 cells, a popular model for investigating diabetes associated β-cell dysfunction. Our experiments indicate that IOS imaging permits simultaneous monitoring of glucose-stimulated physiological responses in multiple cells with high spatial (sub-cellular) and temporal (sub-second) resolution. Rapid IOS image sequences revealed transient optical responses that had time courses tightly correlated with the glucose stimulation.
