In vivo autofluorescence in the biological windows: the role of pigmentation
In vivo autofluorescence in the biological windows: the role of pigmentation
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DOI：
10.1002/jbio.201500271
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发表时间：
2016-10-01
影响因子：
2.8
通讯作者：
Sanz-Rodriguez, Francisco
中科院分区：
文献类型：
--
作者：
del Rosal, Blanca;Villa, Irene;Sanz-Rodriguez, Francisco
在第二生物窗（II-BW，1000-1350 nm）中的小动物深层组织荧光成像受到不期望的红外激发的、红外发射的（900-1700 nm）自发荧光的存在的限制，所述自发荧光的来源、光谱特性和对菌株的依赖性仍然未知。本文系统地研究了五种不同毛色（黑色、灰色、浅绿色、白色和裸色）小鼠的红外自发荧光和激光诱导全身发热。虽然光谱特性和器官自发荧光的大小在小鼠品系之间都没有显着变化，但已经发现毛色强烈地决定了自发荧光强度以及激光诱导的全身加热。包括在这项工作中的结果揭示了小鼠品系作为一个关键参数，必须认真考虑的设计和性能的小动物成像实验的基础上发射红外荧光标记。
Small animal deep-tissue fluorescence imaging in the second Biological Window (II-BW, 1000-1350 nm) is limited by the presence of undesirable infrared-excited, infrare-demitted (900-1700 nm) autofluorescence whose origin, spectral properties and dependence on strains is still unknown. In this work, the infrared autofluorescence and laser-induced whole body heating of five different mouse strains with distinct coat colors (black, grey, agouti, white and nude) has been systematically investigated. While neither the spectral properties nor the magnitude of organ autofluorescence vary significantly between mouse strains, the coat color has been found to strongly determine both the autofluorescence intensity as well as the laser-induced whole body heating. Results included in this work reveal mouse strain as a critical parameter that has to be seriously considered in the design and performance of small animal imaging experiments based on infrared-emitting fluorescent markers.