Overexpression of tumor suppressor protein OSCP1/NOR1 induces ER stress and apoptosis during development of Drosophila melanogaster.
Overexpression of tumor suppressor protein OSCP1/NOR1 induces ER stress and apoptosis during development of Drosophila melanogaster.
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发表时间：
2015-04
影响因子：
5.3
通讯作者：
N. Huu;H. Yoshida;M. Yamaguchi
中科院分区：
文献类型：
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作者：
N. Huu;H. Yoshida;M. Yamaguchi
OSCP 1/NOR 1（organic solute carrier partner 1/oxidored-nitro domain-containing protein 1，有机溶质载体伴侣1/含氧化硝基结构域的蛋白1）已知作为各种有机溶质进入细胞的转运蛋白，并且据报道还作为肿瘤抑制蛋白发挥作用。尽管OSCP 1的过表达已被证明在哺乳动物细胞系中发挥多种作用，但其在活生物体中的生物学意义尚未完全了解。为了探索OSCP 1/NOR 1对发育的影响，我们在果蝇中进行了遗传研究，其特点是过表达其果蝇直向同源物dOSCP 1。dOSCP 1在成年果蝇眼成虫盘中的过表达诱导了粗糙的眼表型，这可能是由于S期进展延迟和caspase依赖性凋亡诱导后的代偿性增殖所致。然而，它似乎没有参与R7感光细胞的分化。我们还发现dOSCP 1的过表达引起唾液腺细胞的内质网应激。这些结果表明dOSCP 1的过表达对果蝇发育过程中的各种生物学过程产生影响。
OSCP1/NOR1 (organic solute carrier partner 1/oxidored-nitro domain-containing protein 1) is known as a transporter of various organic solutes into cells and also is reported to act as a tumor suppressor protein. Although overexpression of OSCP1 has been shown to play multiple roles in mammalian cell lines, its biological significance in living organisms is not fully understood. To explore the effects of OSCP1/NOR1 on development, we performed genetic studies in flies featuring overexpression of its Drosophila orthologue, dOSCP1. Overexpression of dOSCP1 in eye imaginal discs induced a rough eye phenotype in adult flies, likely resulting from a delay in S phase progression and induction of caspase-dependent apoptosis followed by compensatory proliferation. However, it did not appear to be involved in differentiation of R7 photoreceptor cells. We also found that overexpression of dOSCP1 caused endoplasmic reticulum stress in salivary gland cells. These results indicate that overexpression of dOSCP1 exerts effects on various biological processes during Drosophila development.