Induced Pluripotent Stem Cells Meet Genome Editing.
Induced Pluripotent Stem Cells Meet Genome Editing.
复制标题
诱导多能干细胞满足基因组编辑。
DOI：
10.1016/j.stem.2016.04.013
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发表时间：
2016-05-05
期刊：
影响因子：
23.9
通讯作者：
Jaenisch R
中科院分区：
文献类型：
--
作者：
Hockemeyer D;Jaenisch R
单个实验如此具有影响力和及时性，以至于塑造和预测了未来十年的实验，这是极其罕见的。在这里，我们回顾了两项此类实验——人类诱导多能干细胞 (iPSC) 的产生和 CRISPR/Cas9 技术的发展——如何从根本上重塑了我们的生物医学研究、干细胞生物学和人类遗传学方法。我们还将重点介绍 iPSC 和 CRISPR/Cas9 技术所基于的先前知识，因为这一基础工作证明了对解决方案的需求以及这些技术提供的优势，并为其成功奠定了基础。
It is extremely rare for a single experiment to be so impactful and timely that it shapes and forecasts the experiments of the next decade. Here, we review how two such experiments --the generation of human induced pluripotent stem cells (iPSCs) and the development of CRISPR/Cas9 technology-- have fundamentally reshaped our approach to biomedical research, stem cell biology and human genetics. We will also highlight the previous knowledge that iPSC and CRISPR/Cas9 technologies were built on as this groundwork demonstrated the need for solutions and the benefits that these technologies provided, and have set the stage for their success.