Multi-scale structural and mechanical characterisation in bioinspired polyurethane-based pancreatic cancer model
Multi-scale structural and mechanical characterisation in bioinspired polyurethane-based pancreatic cancer model
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DOI：
10.1016/j.jmrt.2021.09.041
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发表时间：
2021-11
期刊：
影响因子：
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通讯作者：
J. Mo;N. Leung;P. Gupta;B. Zhu;Hui-juan Xing;Jiao Zhang;E. Velliou;T. Sui
中科院分区：
文献类型：
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作者：
J. Mo;N. Leung;P. Gupta;B. Zhu;Hui-juan Xing;Jiao Zhang;E. Velliou;T. Sui
本研究采用冷冻铸造法制备了新型仿生聚氨酯（PU）支架，用于胰腺导管腺癌（PDAC）模型。为了重现促进细胞动力学的肿瘤微环境，用细胞外基质（ECM）蛋白对PU支架进行表面修饰，包括胶原蛋白和纤维连接蛋白（Col和FN）。采用基于同步加速器的小角和广角x射线散射（SAXS/WAXS）技术来探测环境力学测试过程中的结构演变。在宏观，纳米和晶格尺度上获得菌株，以研究ECM蛋白和胰腺细胞活性对PU支架的影响。经FN表面改性的PU支架在不同长度尺度上均有明显的力学强化。提出了一种通过增强层间吸收的刚度调制模型来解释多尺度强化机制。了解一系列PU支架的多尺度变形机制为开发一种新的胰腺癌模型提供了机会，用于研究癌症演变和预测药物/治疗的结果。
In this work, novel bioinspired polyurethane (PU) scaffolds were fabricated via freeze casting for PU-based Pancreatic Ductal Adenocarcinoma (PDAC) model. In order to reproduce the tumour micro-environment that facilitates cellular kinetics, the PU scaffolds were surface modified with extracellular matrix (ECM) proteins including collagen and fibronectin (Col and FN). Synchrotron-based small- and wide-angle X-ray scattering (SAXS/WAXS) techniques were applied to probe structural evolution duringin situmechanical testing. Strains at macroscopic, nano-, and lattice scales were obtained to investigate the effects of ECM proteins and pancreatic cell activities to PU scaffolds. Significant mechanical strengthening across length scales of PU scaffolds was observed in specimens surface modified by FN. A model of stiffness modulation via enhanced interlamellar recruitment is proposed to explain the multi-scale strengthening mechanisms. Understanding multi-scale deformation mechanisms of a series of PU scaffolds opens an opportunity in developing a novel pancreatic cancer model for studying cancer evolution and predicting outcomes of drug/treatments.