Occurrence of Cryptosporidium oocysts and Giardia cysts in raw water from the Atibaia River, Campinas, Brazil
Occurrence of Cryptosporidium oocysts and Giardia cysts in raw water from the Atibaia River, Campinas, Brazil
复制标题
DOI：
10.1590/s0036-46652001000200011
复制
发表时间：
2001-03-01
影响因子：
1.9
通讯作者：
Cantusio Neto, Romeu
中科院分区：
文献类型：
--
作者：
Franco, Regina Maura Bueno;Rocha-Eberhardt, Rosangela;Cantusio Neto, Romeu
小隐孢子虫和十二指肠贾第鞭毛虫是水生寄生虫，已引起多起与饮用水相关的胃肠道疾病的爆发。由于缺乏关于巴西东南部水中这些原生动物的存在情况的研究，因此进行了一项调查，以验证阿蒂巴亚河表层原水中是否存在包囊和卵囊。将水样进行膜过滤（3.0μm），并通过（1）刮膜和冲洗膜（RM法）和（2）丙酮溶解（ADM法）进行洗脱。分析微生物和化学参数。通过免疫荧光检查沉淀的等分试样（Meriflor，Meridian Diagnostics，辛辛那提，俄亥俄州）。尽管浑浊度很高，但所有水样均呈隐孢子虫和贾第鞭毛虫阳性。 RM 方法处理的样品比 ADM 技术处理的样品回收率更高。未来工作的目标是评估包囊和卵囊的活力，以确定这一发现的公共卫生意义。
Cryptosporidium parvum and Giardia duodenalis are waterborne parasites that have caused several outbreaks of gastrointestinal disease associated with drinking water. Due to the lack of studies about the occurrence of these protozoa in water in the Southeast of Brazil, an investigation was conducted to verify the presence of cysts and oocysts in superficial raw water of the Atibaia River. The water samples were submitted to membrane filtration (3.0 mum) and elution was processed by (1) scraping and rinsing of membrane (RM method) and (2) acetone-dissolution (ADM method). Microbiologic and chemical parameters were analyzed. Aliquots of the pellets were examined by immunofluorescence (Merifluor, Meridian Diagnostics, Cincinnati, Ohio). All water samples were positive for Cryptosporidium and Giardia, in spite of the high turbidity. Higher recovery rates occurred in samples treated by the RM method than by the ADM technique. The goal for future work is the assessment of viability of cysts and oocysts to determine the public health significance of this finding.