The Genetic Polymorphisms of NLRP3 Inflammasome Associated with T Helper Cells in Patients with Multiple Myeloma
The Genetic Polymorphisms of NLRP3 Inflammasome Associated with T Helper Cells in Patients with Multiple Myeloma
复制标题
多发性骨髓瘤患者辅助性T细胞NLRP3炎症小体基因多态性
DOI：
10.1155/2018/7569809
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发表时间：
2018-01-01
影响因子：
4.1
通讯作者：
Ma, Daoxin
中科院分区：
文献类型：
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作者：
Zhao, Xueyun;Hua, Mingqiang;Ma, Daoxin
多发性骨髓瘤（multiple myeloma，MM）的发病机制尚不清楚，NLRP 3炎性小体在许多疾病的发生发展中的作用越来越受到重视。为探讨NLRP 3炎性体在MM中的作用，我们检测了MM患者中NLRP 3炎性体相关基因（IL-1β、IL-18、CARD 8和NF-κB B）的基因多态性和表达，并探讨其临床意义。本研究进一步探讨了MM患者NLRP 3炎性小体与Th细胞的关系，发现CARD 8-C10 X（rs 2043211）AT基因型与MM的易感性有关，CARD 8-C10 X TT患者临床分期早。3种CARD 8基因型的白细胞计数均呈增高趋势（AA<AT<TT）。与NF-κB-94 ins/del、ATTG ins/ins和ins/del相比，del/del患者骨髓瘤细胞比例最高。IL-18（rs 16944）TT患者的血红蛋白浓度最高（GG<GT<TT）。此外，我们发现CARD 8-C10 X（rs 2043211）或NF-κB-94 ins/del ATG基因型与Th 1的频率密切相关。因此，Th细胞相关的NLRP 3炎性小体基因多态性可能参与了多发性骨髓瘤的发病机制。
The pathogenesis of multiple myeloma (MM) remains unclear and the NLRP3 inflammasome has been more and more recognized in the progression of many diseases. To investigate the role of the NLRP3 inflammasome in MM, we determined the genetic polymorphisms and expression of NLRP3 inflammasome-related genes (IL-1β, IL-18, CARD8, and NF-κB) in MM patients, and explored their clinical relevance. Furthermore, we investigated the relationship of the NLRP3 inflammasome with Th cells in MM. Our study showed that the CARD8-C10X (rs2043211) AT genotype contributed to the susceptibility of MM. CARD8-C10X TT patients had earlier clinical stage. The WBC count in the three CARD8 genotypes showed an increasing trend (AA<AT<TT). Compared with patients with NF-κB-94 ins/del ATTG ins/ins and ins/del, patients with del/del had the highest myeloma cell ratio. Patients with IL-18 (rs16944) TT had the highest hemoglobin concentration (GG<GT<TT). Furthermore, we found that the genotype of CARD8-C10X (rs2043211) or NF-κB-94 ins/del ATTG was closely related to the frequency of Th1. Therefore, the genetic polymorphisms of the NLRP3 inflammasome associated with Th cells might be involved in the pathogenesis of multiple myeloma.