High frequency of human herpesvirus 6 DNA in multiple sclerosis plaques isolated by laser microdissection
High frequency of human herpesvirus 6 DNA in multiple sclerosis plaques isolated by laser microdissection
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DOI：
10.1086/368166
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发表时间：
2003-05-01
影响因子：
6.4
通讯作者：
Jacobson, S
中科院分区：
文献类型：
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作者：
Cermelli, C;Berti, R;Jacobson, S
从多发性硬化症(MS)斑块和正常脑白质(NAWM)的尸检材料中评估了人类疱疹病毒6型(HHV-6)DNA的频率，这些脑组织来自多发性硬化症患者、健康大脑和其他神经系统疾病患者的大脑。用激光显微镜分离福尔马林固定、石蜡包埋的脑组织样本中的特定区域。激光显微解剖脑组织提取DNA，套式聚合酶链式反应扩增HHV-6基因组序列。分析了13例MS患者的44例脑白质标本和MS斑块，13例非MS神经系统疾病患者的46例标本和12例健康对照组的41例标本。44例脑白质瘤标本中，7例(15.9%)HHV-6DNA序列阳性，而MS斑块中37例(57.8%)阳性(P
The frequency of human herpesvirus 6 (HHV-6) DNA was assessed in autopsy material from multiple sclerosis (MS) plaques and normal-appearing white matter (NAWM) from brains of persons with MS, healthy brains, and brains of persons with other neurologic diseases. Specific areas from formalin-fixed, paraffin-embedded brain tissue samples were isolated by laser microscope. DNA was extracted from laser microdissected brain material, and HHV-6 genomic sequences were amplified by nested polymerase chain reaction. We analyzed 44 NAWM samples and 64 MS plaques from 13 patients with MS, 46 samples from 13 patients with non-MS neurologic disorders, and 41 samples from 12 healthy control brains. Of the 44 NAWM samples, 7 (15.9%) were positive for HHV-6 DNA sequences, versus 37 (57.8%) of 64 MS plaques (P