Two Cryptic Species of the Phytophagous Ladybird Beetle Epilachna vigintioctopunctata (Coleoptera: Coccinellidae) Detected by Analyses of Mitochondrial DNA and Karyotypes, and Crossing Experiments
Two Cryptic Species of the Phytophagous Ladybird Beetle Epilachna vigintioctopunctata (Coleoptera: Coccinellidae) Detected by Analyses of Mitochondrial DNA and Karyotypes, and Crossing Experiments
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通过线粒体 DNA 和核型分析以及杂交实验检测到两种隐性植食性瓢虫 Epilachna vigintioctopunctata（鞘翅目：瓢虫科）
DOI：
10.2108/zsj.17.1159
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
H. Katakura
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作者：
N. Kobayashi;Y. Shirai;N. Tsurusaki;K. Tamura;T. Aotsuka;H. Katakura
摘要对来自东亚和东南亚8个地区的17只斑尾章鱼（Fabricius）的线粒体细胞色素c氧化酶I （cytochrome c oxidase I）部分基因序列（645 bp）的分析表明，该种群可分为千叶、东京、那霸、伊里奥莫特、曼谷和吉隆坡、巴东、茂物两个遗传上截然不同的类群。不同组间序列间核苷酸替换数为57 ~ 60个，每组内序列间核苷酸替换数为1 ~ 8个。两组的核型也有明显差异。杂交实验表明，两组间存在较强的交配后屏障：组间杂交所得的卵通常不孵化，而组内杂交所得的卵孵化率超过90%。结果表明，亚洲和澳大利亚的茄类作物的主要害虫，至少由两个生殖隔离的生物物种组成，它们可能分布在不同的地理范围。
Abstract Analyses of a part of mitochondrial cytochrome c oxidase I gene sequences (645 bp) for seventeen individuals of Epilachna vigintioctopunctata (Fabricius) from eight localities in east and southeast Asia revealed that the populations are divided into two genetically distinct groups (Chiba, Tokyo, Naha, Iriomote, Bangkok vs. Kuala Lumpur, Padang, Bogor). The number of nucleotide substitutions between sequences of different groups was 57–60, while that between sequences within each group was 1–8. Karyotypes of the two groups were also distinctly different. Crossing experiments showed that there exist strong postmating barriers between the two groups: eggs obtained from between-group crossings usually did not hatch, whereas more than 90% of eggs from within-group crossings hatched. It is concluded that E. vigintioctopunctata, a notorious pest of solanaceous crops in Asia and Australia, is composed of at least two reproductively isolated biological species that probably occupy different geographic ranges.
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