Generation of Induced Pluripotent Stem Cells from Human Cord Blood
Generation of Induced Pluripotent Stem Cells from Human Cord Blood
复制标题
DOI：
10.1016/j.stem.2009.08.021
复制
发表时间：
2009-10-02
期刊：
影响因子：
23.9
通讯作者：
Martin, Ulrich
中科院分区：
文献类型：
--
作者：
Haase, Alexandra;Olmer, Ruth;Martin, Ulrich
诱导多能干细胞（iPSC）可能是未来再生疗法的理想细胞来源。关于患者特异性iPSC的临床使用的关键问题是在生物体的一生中体细胞（干细胞）中突变的积累。获得性体细胞突变在重编程期间传递到iPSC上，并且可能与细胞功能丧失和癌症形成相关。在这里，我们报告了从脐带血（CB）作为返老还童细胞来源的人iPSC的产生。CBiPSC显示出胚胎干细胞的典型特征，并且可以分化为所有三个胚层的衍生物，包括功能性心肌细胞。对于自体和同种异体iPSC衍生物的未来治疗性生产，CB可以常规地收获用于公共和商业CB库，而没有任何供体风险。CB可以很容易地用于儿科患者，特别是用于患有遗传性疾病或先天性畸形的新生儿。
Induced pluripotent stem cells (iPSCs) may represent an ideal cell source for future regenerative therapies. A critical issue concerning the clinical use of patient-specific iPSCs is the accumulation of mutations in somatic (stem) cells over an organism's lifetime. Acquired somatic mutations are passed onto iPSCs during reprogramming and may be associated with loss of cellular functions and cancer formation. Here we report the generation of human iPSCs from cord blood (CB) as a juvenescent cell source. CBiPSCs show characteristics typical of embryonic stem cells and can be differentiated into derivatives of all three germ layers, including functional cardiomyocytes. For future therapeutic production of autologous and allogeneic iPSC derivatives, CB could be routinely harvested for public and commercial CB banks without any donor risk. CB could readily become available for pediatric patients and, in particular, for newborns with genetic diseases or congenital malformations.