Ovarian Damages Produced by Aerosolized Fine Particulate Matter (PM(2.5)) Pollution in Mice: Possible Protective Medications and Mechanisms.
Ovarian Damages Produced by Aerosolized Fine Particulate Matter (PM(2.5)) Pollution in Mice: Possible Protective Medications and Mechanisms.
复制标题
DOI：
10.4103/0366-6999.207472
复制
发表时间：
2017-06-20
影响因子：
6.1
通讯作者：
Gao GL
中科院分区：
文献类型：
--
作者：
Gai HF;An JX;Qian XY;Wei YJ;Williams JP;Gao GL
在实验中，环境中的气溶胶细颗粒物(PM2.5)与男性生殖毒性有关，并可能对女性产生不利影响。然而，评估阿司匹林、维生素C、维生素E或臭氧对PM2.5毒性影响的保护作用和确切机制的研究很少。本研究旨在探讨阿司匹林、维生素C、维生素E或臭氧对PM2.5染毒雌性小鼠生育力的可能保护作用及其机制。84只ICR小鼠随机分为6组：对照组、PM2.5组、PM2.5+阿司匹林组、PM2.5+维生素C组、PM2.5+维生素E组、PM2.5+臭氧组。PM2.5气管内滴注，隔日1次，连续3周。阿司匹林、维生素C、维生素E口服1次，每日1次，连续3周；臭氧每天1次，腹腔注射，连续3周。采用酶联免疫吸附试验检测血清中抗苗勒氏激素、白介素6、肿瘤坏死因子-α、8-羟基-2‘-脱氧鸟苷(8-α)水平。Western blotting分析卵巢组织中Bcl2、Bax和caspase 3的表达。光镜观察组织结构变化，电子显微镜观察超微结构。结果表明，PM2.5可降低肺泡巨噬细胞激素水平(P<；0.001)，而阿司匹林(P<；0.001)、维生素C(P<；0.001)、维生素E(P=0.001)和臭氧(P=0.002)可缓解这种下降。IL-6、肿瘤坏死因子-α、8-羟色胺、bax/bc-2、caspase-3的变化PM2.5组明显高于对照组(P<0.001)，而PM2.5+阿司匹林组、PM2.5+维生素C组、PM2.5+维生素E组、PM2.5+臭氧组均低于PM2.5组(P<0.001或P<0.05)。PM2.5会对卵巢造成损害，而阿司匹林、维生素C、维生素E和臭氧可以对抗这种损害。其保护机制可能与其钝化PM2.5引起的炎症和氧化应激，进而抑制细胞凋亡调节蛋白的表达，降低卵巢细胞凋亡率有关。
Ambient aerosol fine particulate matter (PM2.5) is associated with male reproductive toxicity in experiments and may have adverse effects in the female. However, studies evaluating the protective effects and precise mechanisms of aspirin, Vitamin C, Vitamin E, or ozone against toxic effects of PM2.5 are sparse. This study was conducted to investigate the possible protective effects and mechanisms of aspirin, Vitamin C, Vitamin E, or ozone on fertility in female mice treated with PM2.5. Eighty-four ICR mice were divided into six groups: control group, PM2.5 group, PM2.5 + aspirin group, PM2.5 + Vitamin C group, PM2.5 + Vitamin E group, and PM2.5 + ozone group. PM2.5 was given by intratracheal instillation every 2 days for 3 weeks. Aspirin, Vitamin C, and Vitamin E were given once a day by oral gavage for 3 weeks, and ozone was administered by intraperitoneal injection once a day for 3 weeks. The levels of anti-Müllerian hormone (AMH), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and 8-hydroxy-2’-deoxyguanosine (8-OHdG) were measured using enzyme-linked immunosorbent assay. Western blotting analysis was used to analyze the expressions of Bcl-2, Bax, and caspase-3 in ovaries. Changes in histological structure were examined by light microscope and electron microscopy was used to detect ultramicrostructure. The results demonstrated that PM2.5 decreased AMH levels (P < 0.001); however, aspirin (P < 0.001), Vitamin C (P < 0.001), Vitamin E (P = 0.001), and ozone (P = 0.002) alleviated the decrease. Changes of IL-6, TNF-α, 8-OHdG, Bax/Bcl-2, and caspase-3 in PM2.5 group were increased compared to control group (P < 0.001), while in PM2.5 + aspirin, PM2.5 + Vitamin C, PM2.5 + Vitamin E, and PM2.5 + ozone groups, they were statistically decreased compared to PM2.5 group (P < 0.001 or P < 0.05). PM2.5 cause the damage of ovaries, and aspirin, Vitamin C, Vitamin E, and ozone antagonizes the damage. The protective mechanism is probably due to its ability to blunt the inflammatory and oxidative stress caused by PM2.5, which subsequently suppressing the expression of apoptotic regulatory protein and reducing the incidence of ovary apoptosis.