Distinct transcription factors bind specifically to two regions of the human histone H4 promoter.
Distinct transcription factors bind specifically to two regions of the human histone H4 promoter.
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不同的转录因子特异性结合人类组蛋白 H4 启动子的两个区域。
DOI：
10.1073/pnas.83.19.7241
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发表时间：
1986
影响因子：
11.1
通讯作者：
Heintz,N
中科院分区：
文献类型：
--
作者：
Dailey,L;Hanly,SM;Roeder,RG;Heintz,N
在同步化 S 期 HeLa 细胞制备的核提取物中鉴定出两种与人组蛋白 H4 启动子不同区域特异性结合的蛋白质。 H4 启动子突变体的竞争实验和 DNase 保护测定（“足迹”）表明，这些因子与 H4 启动子的区域结合，这对于体外最大表达至关重要。这些因子之一 (H4TF-1) 与 H4 帽位点上游 -80 和 -110 碱基对之间的序列结合，而另一个 (H4TF-2) 与紧邻“TATA”同源性上游的 H4 亚型特异性序列元件结合。这些活性都不能有效地结合任何其他组蛋白基因亚型或猿猴病毒 40 DNA。 H4TF-1 与 pHu4A 组蛋白 H4 启动子远端区域的结合被本实验室克隆的 6 个人类组蛋白 H4 基因中的 4 个以不同的效率竞争性抑制，而 6 个 H4 基因中的 5 个则表现出对 H4TF-2 结合的有效竞争。由于这两个因子都与 pHu4A 组蛋白 H4 启动子的重要区域结合，并且可以与几种不同的人类 H4 基因结合，因此我们相信它们对于基因的最大转录很重要，并且它们可能参与其在细胞周期中的调节表达。
Two proteins specifically binding to separate regions of the human histone H4 promoter were identified in nuclear extracts prepared from synchronized S-phase HeLa cells. Competition experiments with H4 promoter mutants and DNase protection assays ("footprinting") demonstrate that these factors bind to regions of the H4 promoter that are essential for maximal expression in vitro. One of these factors (H4TF-1) binds to sequences between -80 and -110 base pairs upstream of the H4 cap site, whereas the other (H4TF-2) binds to the H4 subtype-specific sequence element immediately upstream from the "TATA" homology. Neither of these activities can efficiently bind to any of the other histone gene subtypes or simian virus 40 DNA. Binding of H4TF-1 to the distal region of the pHu4A histone H4 promoter is inhibited competitively with varying efficiency by four of six human histone H4 genes cloned in this laboratory, whereas efficient competition for binding of H4TF-2 is exhibited by five of the six H4 genes. Since both of these factors bind to significant regions of the pHu4A histone H4 promoter and can be bound by several different human H4 genes, we believe that they are important for maximal transcription of the gene and that they may be involved in its regulated expression during the cell cycle.