[Evaluation of the ELISA and the enhanced chemiluminescence immunoassay in use to determine the serum markers for liver fibrosis].
[Evaluation of the ELISA and the enhanced chemiluminescence immunoassay in use to determine the serum markers for liver fibrosis].
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DOI：
10.3760/cma.j.issn.1003-9279.2009.01.025
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发表时间：
2009-02
期刊：
影响因子：
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通讯作者：
Jing-xia Guo;Hong-bin Ma;Yong-li Li;Jun Xu;Li-hua Yang;Jia-bin Shi;Bo-an Li;Aixia Liu;Xiao-xi Li;Bo-an Li;Y. Mao
中科院分区：
文献类型：
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作者：
Jing-xia Guo;Hong-bin Ma;Yong-li Li;Jun Xu;Li-hua Yang;Jia-bin Shi;Bo-an Li;Aixia Liu;Xiao-xi Li;Bo-an Li;Y. Mao
目的评价酶联免疫吸附法（ELISA）和增强化学发光免疫分析法（ECLIA）检测血清透明质酸（HA）、层粘连蛋白（LN）、IV型胶原（IV-C）和III型前胶原（PC III）的方法。方法对253例慢性B型肝炎患者分别采用电化学发光法和酶联免疫吸附法检测4种肝纤维化血清标志物，并与病理结果进行比较。结果ELISA和ECLIA检测结果均能反映患者从肝炎到肝硬化肝纤维化的逐渐加重过程。与ELISA法相比，ECLIA法检测结果与病理结果有较好的相关性。结论电化学发光法检测肝纤维化血清标志物能更好地反映肝纤维化状态。
OBJECTIVE To evaluate the detection method of ELISA and Enhanced Chemiluminescence Immunoassay (ECLIA) in use to determine serum hyaluronate acid (HA), laminin (LN), type IV collagen (IV-C) and type III procollagen (PC III). METHODS 253 patients with chronic hepatitis B were determined the four liver fibrosis serum markers with both the ECLIA and ELISA, and then compared with pathology results separately. RESULTS Both the detection results of ELISA and ECLIA can reflect that the patient's liver fibrosis from hepatitis to liver cirrhosis aggravated gradually. Compared with ELISA, the results of ECLIA and pathology have a better correlation. CONCLUSIONS The detection of four liver fibrosis serum markers by ECLIA could indicate the better the response of the state of live fibrosis.