Study on the interaction between aglycon of daunorubicin and calf thymus DNA by spectroscopy
Study on the interaction between aglycon of daunorubicin and calf thymus DNA by spectroscopy
复制标题
光谱法研究柔红霉素苷元与小牛胸腺DNA相互作用
DOI：
10.1016/j.molstruc.2011.06.024
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发表时间：
2011-08-24
影响因子：
3.8
通讯作者：
Xing, Weiwei
中科院分区：
文献类型：
--
作者：
Cui, Fengling;Huo, Ruina;Xing, Weiwei
用紫外吸收光谱和荧光光谱研究了柔红霉素苷元（DNR-A）与小牛胸腺DNA （ctDNA）的相互作用。在ctDNA存在下，DNR-A的吸收光谱出现了异色效应。DNR-A的荧光强度随ctDNA的加入而降低。不同温度下的Stern-Volmer图证明了其猝灭机制为静态猝灭。DNR-A与ctDNA在301、310和320 K下的结合常数分别为6.203、4.782、3.334 × 10(4) L mol（-1）。热力学参数也得到了，这表明疏水力可能在DNR-A与ctDNA的结合中起主要作用。随着DNR-A的加入，ctDNA的相对粘度增加，同时ctDNA在DNR-A存在下的熔融温度升高。离子强度的影响表明NaCl的加入不影响DNR-A与ctDNA的结合。此外，荧光猝灭研究表明，与ctDNA结合的DNR-A的K-SV值低于游离DNR-A。荧光偏振和变性DNA的研究也提供了强有力的证据，证明DNR-A分子嵌入在ctDNA的碱基对之间。所有结果均支持DNR-A与ctDNA的主要结合方式为插层。这些研究有助于更好地了解DNR-A与ctDNA的相互作用，这将有助于设计新的DNA靶向药物。(C) 2011 Elsevier B.V.版权所有
The interaction between aglycon of daunorubicin (DNR-A) and calf thymus DNA (ctDNA) was investigated by UV absorption spectra and fluorescence spectra. Hypochromic effect was founded in the absorption spectra of DNR-A in the presence of ctDNA. The fluorescence intensity of DNR-A was decreased with the addition of ctDNA. Stern-Volmer plots at different temperatures proved that the quenching mechanism was a static quenching type. The binding constants of DNR-A with ctDNA at 301, 310 and 320 K were 6.203, 4.782, 3.334 x 10(4) L mol(-1), respectively. The thermodynamic parameters were also obtained, which suggested that hydrophobic force might play a major role in the binding of DNR-A to ctDNA. The relative viscosity of ctDNA increased with the addition of DNR-A, at the same time the value of melting temperature of ctDNA increased in the presence of DNR-A. Effect of ionic strength showed that the addition of NaCl could not affect the binding of DNR-A and ctDNA. In addition, fluorescence quenching study indicated that K-SV value for the bound DNR-A with ctDNA was lower than the free DNR-A. Fluorescence polarization and denatured DNA studies also applied strong evidences that DNR-A molecule was intercalated between the base pairs of ctDNA. All the results supported that the major binding mode of DNR-A and ctDNA was intercalation. These studies were helpful for a better understanding in the interaction of DNR-A with ctDNA, which should be beneficial to the design of new DNA targeted drugs. (C) 2011 Elsevier B.V. All rights reserved.