Bacterial diversity in cucumber (Cucumis sativus) rhizosphere in response to salinity, soil pH, and boron
Bacterial diversity in cucumber (Cucumis sativus) rhizosphere in response to salinity, soil pH, and boron
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DOI：
10.1016/j.soilbio.2009.11.033
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发表时间：
2010-04-01
影响因子：
9.7
通讯作者：
Suarez, D.
中科院分区：
文献类型：
--
作者：
Ibekwe, A. M.;Poss, J. A.;Suarez, D.
在微生物选择过程中，土壤盐度是微生物群落与环境胁迫相关的一个主要因素，因为胁迫会减少细菌多样性。在加利福尼亚州的圣华金河谷(Sjv)，由于咸水排放水的再利用，盐分增加，从而导致作物产量下降的问题是人们主要关注的问题。在一个由24个植物生长单元组成的封闭、循环的体积蒸渗仪系统中，研究了盐分、硼和pH对黄瓜(黄瓜L cv.)根际和非根际微生物组成的互作效应。塞米尼斯涡轮混合动力车)。VLS中的植物是从个别水库灌溉的，这些水库含有改良的一半强度的霍格兰营养液，并结合了盐度、硼(B)和pH处理。结果表明，盐度和pH对植物生长的影响最大，在弱酸性条件下，硼对植物生长的影响较大。从根际和非根际样品中提取细菌总DNA，在真细菌核糖体小亚基RNA基因的V3区扩增出一条236个碱基的DNA片段。对16S rRNA及其产物进行变性梯度凝胶电泳(DGGE)和测序。细菌多样性分析表明，在黄瓜生长的第一周，盐度、硼和pH对根际细菌数量的影响较大，随着植株的生长，影响逐渐减小。然而，盐度-B-pH对植物生物量没有交互作用，但在研究的第七周，对根际异养细菌的数量和物种丰富度和多样性产生了影响。这些表明，盐度-B-pH相互作用的影响可能首先影响微生物，然后才是植物，并可能对土壤质量造成长期影响。爱思唯尔有限公司出版。
Soil salinity is a major factor relating microbial communities to environmental stress in the microbial selection process as stress can reduce bacterial diversity. In the San Joaquin Valley (SJV) of California, the problem of increasing salinity and consequently, decreasing crop productivity, due to reuse of saline drainage water are major concerns. An experiment was conducted in a closed, recirculating volumetric lysimeter system (VLS) consisting of 24 experimental plant growth units to determine the interactive effects of salinity, boron and pH on rhizosphere and non-rhizosphere microbial composition of cucumber (Cucumis sativus L cv. Seminis Turbo hybrid). Plants in the VLS were irrigated from individual reservoirs containing a modified half-strength Hoagland's nutrient solution combined with salinity, boron (B), and pH treatments. The results indicated that salinity and pH were the most influential factors affecting the growth of plants and the effect of boron on the plant was more severe under slightly acidic conditions. Total bacterial DNA was extracted from rhizosphere and non-rhizosphere samples, and a 236-bp DNA fragment in the V3 region of the small subunit ribosomal RNA genes of eubacteria was amplified. The 16S rRNA and the products were subjected to denaturing gradient gel electrophoresis (DGGE) and sequencing. Analyses of bacterial diversity showed that the effects of salinity, boron, and pH were more severe on the rhizosphere bacterial population during the first week of growing cucumber, with decreasing impacts with plant growth. However, there was no salinity-B-pH interaction effects on plant biomass, but the effects were seen in the number of heterotrophic bacteria in the rhizosphere and on species richness and diversity during week seven of the study. These suggest that the effects of salinity-B-pH interactions may influence microorganisms first before plants and may pose long term effects on soil quality. Published by Elsevier Ltd.