Effects of ? -amylase and glucoamylase on the characterization and function of maize porous starches
Effects of ? -amylase and glucoamylase on the characterization and function of maize porous starches
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DOI：
10.1016/j.foodhyd.2021.106661
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发表时间：
2021-02-16
影响因子：
10.7
通讯作者：
Xie, Jianhua
中科院分区：
文献类型：
--
作者：
Han, Xiuying;Wen, Huiliang;Xie, Jianhua
为了克服淀粉有限的表面积，提高淀粉的吸附能力，多孔淀粉在各个领域得到了广泛的应用。在本研究中，？采用-淀粉酶（AM）、葡萄糖淀粉酶（GAM）和混合酶（ME）制备不同类型的多孔玉米淀粉，研究了多孔淀粉的微观结构、颗粒形态、吸附和糊化特性。扫描电镜显示不同类型酶及其活性的多孔结构具有不同的孔径分布和孔面积；AM加工的孔最大，GAM加工的孔最深，ME加工的孔又大又深。酶修饰对吸附量的影响显著，而GAM对吸附量的影响更大。通过x射线衍射和傅里叶变换红外光谱分析得出多孔淀粉具有较高的相对结晶度。ma处理的淀粉具有更高的溶解度，而与天然淀粉相比，两种处理的膨胀能力、峰值粘度、最终粘度、分解和挫折都有所降低。结果表明，多孔玉米淀粉可以根据酶的作用模式提供不同的功能。该酶的种类及其酶活性可用于制备理想腔孔径淀粉颗粒，扩大了其应用范围。
To overcome the limited surface area of starch and improve the adsorption capacity of starch, porous starch has been widely produced in various fields. In this study, the different enzyme activities of ?-amylase (AM), glucoamylase (GAM), and mixed enzyme (ME) were used to prepare different types of porous maize starch, and the microstructure, particle morphology, adsorption, and gelatinization characteristics of the porous starch were studied. Scanning electron microscopy showed porous structures with diverse pore size distribution and pore area depending on the enzyme type and its activities; AM produced the largest holes, GAM produced the deepest holes, and ME produced large and deep holes. Adsorptive capacity was significantly affected by enzymatic modification being greater influenced by GAM. The porous starches had higher relative crystallinity as deduced from X-ray diffraction and Fourier transform infrared spectroscopy. The MA-treated starch had higher solubility whereas swelling capacity, peak viscosity, final viscosity, breakdown, and setback of both treatments were decreased compared to the native starch. The results indicate porous maize starch can provide different functions according to the action pattern of enzymes. The type of enzyme and its enzyme activity can be used to produce the ideal cavity and pore size starch granules, which expands its application scope.