Chloroplast culture. IX. Chlorophyll(ide) a biosynthesis in vitro at rates higher than in vivo.
Chloroplast culture. IX. Chlorophyll(ide) a biosynthesis in vitro at rates higher than in vivo.
复制标题
叶绿体培养。
DOI：
10.1016/0006-291x(82)91133-0
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
C. Rebeiz
中科院分区：
文献类型：
--
作者：
H. Daniell;C. Rebeiz
叶绿素a是植物色素，在自然界中催化太阳能转化为化学能。用激动素和赤霉酸在黑暗中预处理黄化黄瓜子叶，观察到从这些组织中分离的质体，在辅因子强化的培养基中，在重复的光-暗条件下培养，能够以约两倍于在活体绿化组织中观察到的最高速率从外源δ-氨基乙酰丙酸合成叶绿素（ide）a。
Chlorophyll a is the plant pigment which in nature catalyzes the conversion of solar energy into chemical energy. By pretreating etiolated cucumber cotyledons with kinetin and gibberellic acid in the dark, it was observed that the plastids which were isolated from such tissues, and incubated in a cofactor-fortified medium, under a repetitive light-dark regime, were capable of synthesizing chlorophyll (ide) a from exogenous δ-aminolevulinic acid at a rate about twice as high as the highest rates observable in greening tissues in vivo.