Effects of voluntary exercise on blood pressure, angiotensin II, aldosterone, and renal function in two-kidney, one-clip hypertensive rats.
Effects of voluntary exercise on blood pressure, angiotensin II, aldosterone, and renal function in two-kidney, one-clip hypertensive rats.
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DOI：
10.2147/ibpc.s147122
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发表时间：
2017
影响因子：
2.2
通讯作者：
Rossi NF
中科院分区：
文献类型：
--
作者：
Waldman BM;Augustyniak RA;Chen H;Rossi NF
自发动态运动促进两肾一夹（2K-1C）高血压大鼠交感神经抑制并降低动脉压。肾交感神经刺激肾素分泌，增加肾小管钠重吸收。我们假设2K-1C大鼠每天自愿进行转轮跑步运动会降低平均动脉压（MAP）、血浆血管紧张素II（Ang II）和醛固酮，并使尿钠和钾排泄正常化，而与肾小球滤过率（GFR）的变化无关。对五周龄雄性Sprague道利大鼠进行假夹闭（Sham）或右肾动脉夹闭（2 K-1C）。将大鼠随机分配至标准笼舍（SED）或带转轮的笼舍（EX）。12周后，大鼠被分配到收集主动脉血用于测量Ang II和醛固酮或评估菊粉清除率和排泄功能。两个EX组的跑步距离相当。2K-1C EX组MAP低于2K-1C SED组（P<0.05）。2K-1C SED大鼠血浆Ang II和醛固酮显著升高，2K-1C EX大鼠血浆Ang II和醛固酮显著降低至与Sham SED或Sham EX大鼠相似的水平。两个2K-1C组中的夹取肾脏重量显著较低，但四个组中的右肾和左肾的GFR和尿流率没有差异。2K-1C SED大鼠未夹肾的总钠排泄率和部分钠排泄率均高于Sham组（P<0.05）。2K-1C EX大鼠的值与Sham组相似。钾排泄量大于钠排泄量。这些研究表明，在2K-1C大鼠自愿动态运动降低血浆血管紧张素II和醛固酮，这有助于降低动脉压，而不会对GFR产生有害影响。对钠排泄的影响强调了压力性尿钠排泄的影响，尽管在久坐的2K-1C大鼠中血浆Ang II和醛固酮升高。相比之下，钾排泄主要受循环醛固酮和远端钠输送的调节。
Spontaneous dynamic exercise promotes sympathoinhibition and decreases arterial pressure in two-kidney, one-clip (2K-1C) hypertensive rats. Renal sympathetic nerves stimulate renin secretion and increase renal tubular sodium reabsorption. We hypothesized that daily voluntary wheel running exercise by 2K-1C rats will decrease mean arterial pressure (MAP), plasma angiotensin II (Ang II), and aldosterone as well as normalize urinary sodium and potassium excretion independent of changes in glomerular filtration rate (GFR). Five-week-old male Sprague Dawley rats underwent sham clipping (Sham) or right renal artery clipping (2K-1C). Rats were randomized to standard caging (SED) or cages with running wheels (EX). After 12 weeks, rats were assigned to either collection of aortic blood for measurement of Ang II and aldosterone or assessment of inulin clearances and excretory function. Running distances were comparable in both EX groups. MAP was lower in 2K-1C EX vs 2K-1C SED rats (P<0.05). Plasma Ang II and aldosterone were significantly higher in 2K-1C SED rats and decreased in 2K-1C EX rats to levels similar to Sham SED or Sham EX rats. Clipped kidney weights were significantly lower in both 2K-1C groups, but GFR and urine flow rates were no different from right and left kidneys among the four groups. Total and fractional sodium excretion rates from the unclipped kidney of 2K-1C SED rats were higher vs either Sham group (P<0.05). Values in 2K-1C EX rats were similar to the Sham groups. Potassium excretion paralleled sodium excretion. These studies show that voluntary dynamic exercise in 2K-1C rats decreases plasma Ang II and aldosterone, which contribute to the lower arterial pressure without deleterious effects on GFR. The effects on sodium excretion underscore the impact of pressure natriuresis despite elevated plasma Ang II and aldosterone in sedentary 2K-1C rats. In contrast, potassium excretion is primarily regulated by circulating aldosterone and distal sodium delivery.