CALIBRATION OF METHODS FOR DETERMINING NUMBERS OF DORSAL-ROOT GANGLION-CELLS
CALIBRATION OF METHODS FOR DETERMINING NUMBERS OF DORSAL-ROOT GANGLION-CELLS
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DOI：
10.1016/0165-0270(90)90123-w
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发表时间：
1990-12-01
影响因子：
3
通讯作者：
KLEIN, CM
中科院分区：
文献类型：
--
作者：
COGGESHALL, RE;LAFORTE, R;KLEIN, CM
初级传入神经元的数量是初级感觉系统组织的重要基础。 然而，一个主要的困难是，不同的研究人员不同意神经元计数。 我们认为，问题在于用于确定这些数字的各种方法并不能提供相同的结果。 因此，为了确保方法提供准确的计数，校准是必要的。 为了做到这一点，神经节细胞的真实数量通过连续重建四个大鼠腰背根神经节细胞群体的重要部分来确定。 然后使用6种常用的神经元计数方法来确定这些相同群体的神经元数量。 数据表明，经验的方法，使用本文中建议的修改，估计所需的精度，而其他5个神经元的数量没有。 因此，在所测试的计数程序中，推荐使用经验方法。 如果要使用其他方法，也应对其进行校准。
Numbers of primary afferent neurons underlie important generalizations concerning the organization of primary sensory systems. A major difficulty, however, is that different investigators do not agree on the neuronal counts. The problem, in our opinion, is that the various methods used to determine these numbers do not provide the same results. Thus to be certain that a method provides accurate counts, calibration is necessary. To do this, true numbers of ganglion cells were determined by serially reconstructing significant parts of four rat lumbar dorsal root ganglion cell populations. Then 6 commonly used methods of counting neurons were used to determine neuron numbers for these same populations. The data indicate that the empirical method, using the modifications recommended in this paper, estimates numbers of neurons with the needed accuracy whereas the other 5 do not. Thus, of the tested counting procedures, the empirical method is recommended. If other methods are to be used, they should also be calibrated.