New tools for quantitative phosphoproteome analysis
New tools for quantitative phosphoproteome analysis
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DOI：
10.1006/bbrc.2001.6275
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发表时间：
2002-01-25
影响因子：
3.1
通讯作者：
Veenstra, TD
中科院分区：
文献类型：
--
作者：
Conrads, TP;Issaq, HJ;Veenstra, TD
分析方法的最新进展，特别是在质谱领域，使蛋白质组学领域成为生物科学的前沿。蛋白质组学的最终目标是表征在特定条件下细胞内表达的蛋白质，由于细胞内蛋白质群体的复杂性和动态性，这是令人生畏的。虽然大部分的努力都集中在开发方法，以确定表达的蛋白质，翻译后修饰的识别是同样重要的全面的蛋白质组表征。在所有已知的翻译后修饰中，磷酸化可以说在细胞稳态的背景下起着最大的作用。这篇综述讨论了一些最近取得的进展，不仅在技术的发展，以确定，但也定量测定磷酸化位点。
Recent advances in analytical methods, particularly in the area of mass spectrometry, have brought the field of proteomics to the forefront in biological science. The ultimate goal of proteomics-to characterize proteins expressed within a cell under a specific set of conditions-is daunting due to the complexity and dynamic nature the of protein population within the cell. While much of the effort has focused on developing methods to identify expressed proteins, the identification of post-translational modifications is equally important for comprehensive proteome characterization. Of all the known post-translational modifications, phosphorylation arguably plays the largest role in the context of cellular homeostasis. This review discusses some of the recent progress made in the development of techniques not only to identify, but also to quantitatively determine sites of phosphorylation.