Lasing within Live Cells Containing Intracellular Optical Microresonators for Barcode-Type Cell Tagging and Tracking
Lasing within Live Cells Containing Intracellular Optical Microresonators for Barcode-Type Cell Tagging and Tracking
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DOI：
10.1021/acs.nanolett.5b02491
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发表时间：
2015-08-01
期刊：
影响因子：
10.8
通讯作者：
Gather, Make C.
中科院分区：
文献类型：
--
作者：
Schubert, Marcel;Steude, Anja;Gather, Make C.
我们报告了一种完全嵌入单个活细胞内的激光器。通过利用细胞的自然内吞作用，我们将荧光回音壁模式（WGM）微谐振器引入细胞质中。当用纳焦耳光脉冲泵浦时，细胞内部会产生绿色激光发射。我们的方法可以应用于不同的细胞类型，并且带有微谐振器的细胞在标准条件下可保持数周的活性。激光光谱的特性为每个细胞提供了条形码型标签，能够唯一地识别和跟踪单个迁移细胞。细胞的自持激光为新形式的细胞追踪、细胞内传感和自适应成像铺平了道路。
We report on a laser that is fully embedded within a single live cell. By harnessing natural endocytosis of the cell, we introduce a fluorescent whispering gallery mode (WGM) microresonator into the cell cytoplasm. On pumping with nanojoule light pulses, green laser emission is generated inside the cells. Our approach can be applied to different cell types, and cells with microresonators remain viable for weeks under standard conditions. The characteristics of the lasing spectrum provide each cell with a barcode-type label which enables uniquely identifying and tracking individual migrating cells. Self-sustained lasing from cells paves the way to new forms of cell tracking, intracellular sensing, and adaptive imaging.