Optical coherence elastography of engineered and developing tissue
Optical coherence elastography of engineered and developing tissue
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DOI：
10.1089/ten.2006.12.63
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发表时间：
2006-01-01
影响因子：
--
通讯作者：
Boppart, SA
中科院分区：
文献类型：
--
作者：
Ko, HJ;Tan, W;Boppart, SA
生物力学弹性特性是用于表征体内和体外组织的许多变量之一。由于这些特性在很大程度上取决于组织的微观和宏观结构组织，因此了解组织对外力或疾病过程做出反应时的机械特性和变化至关重要。使用一种称为光学相干弹性成像（OCE）的新技术，我们绘制了空间分布的机械位移和应变在一个代表性的模型的发展中，工程组织的细胞开始增殖和附着在三维胶原蛋白基质。OCE也在非洲爪蟾（非洲青蛙）蝌蚪的复杂发育组织中进行。位移进行量化的互相关算法压缩前和压缩后的图像，这是使用光学相干断层扫描（OCT）采集。在10天的发育期内获得工程组织的图像，以观察各个区域的相对应变差异。OCE能够区分应变随时间的变化，这与组织学观察证实的细胞增殖和基质沉积相对应。通过以微米分辨率对刚度的区域变化进行解剖学映射，可能会对工程组织和天然组织发展复杂结构的复杂过程提供新的见解。
Biomechanical elastic properties are among the many variables used to characterize in vivo and in vitro tissues. Since these properties depend largely on the micro- and macroscopic structural organization of tissue, it is crucial to understand the mechanical properties and the alterations that occur when tissues respond to external forces or to disease processes. Using a novel technique called optical coherence elastography (OCE), we mapped the spatially distributed mechanical displacements and strains in a representative model of a developing, engineered tissue as cells began to proliferate and attach within a three-dimensional collagen matrix. OCE was also performed in the complex developing tissue of the Xenopus laevis (African frog) tadpole. Displacements were quantified by a cross-correlation algorithm on pre- and postcompression images, which were acquired using optical coherence tomography (OCT). The images of the engineered tissue were acquired over a 10-day development period to observe the relative strain differences in various regions. OCE was able to differentiate changes in strain over time, which corresponded with cell proliferation and matrix deposition as confirmed with histological observations. By anatomically mapping the regional variation of stiffness with micron resolution, it may be possible to provide new insight into the complex process by which engineered and natural tissues develop complex structures.