Identification, molecular characterization and expression of JAZ genes in Lycoris aurea
Identification, molecular characterization and expression of JAZ genes in Lycoris aurea
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DOI：
10.1371/journal.pone.0230177
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发表时间：
2020-03
期刊：
影响因子：
3.7
通讯作者：
Peng Wang;S. Yu;Xiaokang Han;Junya Xu;Qingyuan He;Sheng Xu;Ren Wang
中科院分区：
文献类型：
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作者：
Peng Wang;S. Yu;Xiaokang Han;Junya Xu;Qingyuan He;Sheng Xu;Ren Wang
茉莉酸盐（Jasmonates, JAs）是调控植物生长发育、胁迫反应和次生代谢的关键植物激素。有报道称，用JAs处理可以提高香堇科植物中香堇科生物碱的含量。茉莉酸锌指花序分生系统结构域（Jasmonate ZIM， zinc-finger花序分生系统）结构域（JAZ）蛋白是JA信号处理的关键成分。然而，在石蒜属中尚未发现JAZ蛋白。本研究从石蒜中鉴定并克隆了7个差异表达的JAZ基因（即LaJAZ1-LaJAZ7）。生物信息学分析表明，这7个LaJAZ蛋白含有ZIM结构域和JA-associated (Jas，也称为CCT_2) motif。定量逆转录聚合酶链反应（qRT-PCR）分析显示，这些LaJAZ基因在金黄色葡萄球菌组织中表现出不同的表达模式，且大部分在茉莉酸甲酯（MeJA）处理下可诱导表达。亚细胞定位实验表明，LaJAZ蛋白定位于细胞核或细胞质中。此外，LaJAZ蛋白可以相互作用形成同型二聚体和/或异源二聚体。本研究结果可为进一步研究LaJAZ基因的功能，特别是对金莲属植物次生代谢产物（包括Amaryllidaceae生物碱）的潜在调控机制提供参考。
Jasmonates (JAs) are key phytohormones involved in regulation of plant growth and development, stress responses, and secondary metabolism. It has been reported that treatments with JAs could increase the contents of Amaryllidaceae alkaloids in Amaryllidaceae plants. Jasmonate ZIM (zinc-finger inflorescence meristem) domain (JAZ) proteins are key components in JA signal processes. However, JAZ proteins have not been characterized in genus Lycoris. In this study, we identified and cloned seven differentially expressed JAZ genes (namely LaJAZ1–LaJAZ7) from Lycoris aurea. Bioinformatic analyses revealed that these seven LaJAZ proteins contain the ZIM domain and JA-associated (Jas, also named CCT_2) motif. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed that these LaJAZ genes display different expression patterns in L. aurea tissues, and most of them are inducible when treated with methyl jasmonate (MeJA) treatment. Subcellular localization assay demonstrated that LaJAZ proteins are localized in the cell nucleus or cytoplasm. In addition, LaJAZ proteins could interact with each other to form homodimer and/or heterodimer. The findings in this study may facilitate further functional research of the LaJAZ genes, especially the potential regulatory mechanism of plant secondary metabolites including Amaryllidaceae alkaloids in L. aurea.