Intracellular temperature mapping with fluorescence-assisted photoacoustic-thermometry
Intracellular temperature mapping with fluorescence-assisted photoacoustic-thermometry
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DOI：
10.1063/1.4807140
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发表时间：
2013-05-13
影响因子：
4
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Gao, Liang;Zhang, Chi;Wang, Lihong V.
测量细胞内温度对于了解许多细胞功能至关重要，但仍然具有挑战性。在这里，我们提出了一种技术-荧光辅助光声测温（FAPT）-细胞内温度映射的应用。为了证明FAPT，我们监测的HeLa细胞亚度（0.7摄氏度）的温度分辨率和亚微米（0.23 μ m）的空间分辨率在1 kHz的采样率的细胞内温度分布。与传统的基于荧光的方法相比，FAPT具有将常规荧光探针转化为浓度和激发无关的温度传感器的独特能力，将大量市售通用荧光探针带入细胞内温度传感领域。(C)2013 AIP Publishing LLC.
Measuring intracellular temperature is critical to understanding many cellular functions but still remains challenging. Here, we present a technique-fluorescence-assisted photoacoustic thermometry (FAPT)-for intracellular temperature mapping applications. To demonstrate FAPT, we monitored the intracellular temperature distribution of HeLa cells with sub-degree (0.7 degrees C) temperature resolution and sub-micron (0.23 mu m) spatial resolution at a sampling rate of 1 kHz. Compared to traditional fluorescence-based methods, FAPT features the unique capability of transforming a regular fluorescence probe into a concentration-and excitation-independent temperature sensor, bringing a large collection of commercially available generic fluorescent probes into the realm of intracellular temperature sensing. (C) 2013 AIP Publishing LLC.