Induction of metallothionein isoforms in cultured bovine aortic endothelial cells exposed to cadmium
Induction of metallothionein isoforms in cultured bovine aortic endothelial cells exposed to cadmium
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DOI：
10.2131/jts.45.801
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发表时间：
2020-01-01
影响因子：
2
通讯作者：
Kaji, Toshiyuki
中科院分区：
文献类型：
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作者：
Fujie, Tomoya;Ozaki, Yusuke;Kaji, Toshiyuki
金属硫蛋白 (MT) 是一种诱导蛋白，具有针对镉、锌和铜等重金属的细胞保护活性。 MT-1 和 MT-2 是由重金属诱导并结合重金属的 MT 亚型。牛主动脉内皮细胞含有三种类型的MT基因。即MT-1A。 MT-1E、MT-2A；然而，这些 MT 亚型的相关蛋白表达尚未确定。在本研究中，使用高效液相色谱-电感耦合等离子体质谱系统鉴定了经氯化镉处理的细胞中MT亚亚型蛋白的表达。结果表明：（1）镉对MT-1A转录诱导的敏感性明显高于MT-1E/2A； (2) MT-1A 和 MT-2A 蛋白是镉诱导的主要 MT 亚型，(3) MT-1 和 MT-2 对抗镉细胞毒性的功能可能存在差异，尽管 MT 亚型在细胞中的实际作用并不明显。这是第一项表明镉对血管内皮细胞中 MT 蛋白异构体的差异诱导的研究。
Metallothionein (MT) is an inducible protein with cytoprotective activity against heavy metals such as cadmium, zinc, and copper. MT-1 and MT-2 are the isoforms of MT induced by and bind the heavy metals. Bovine aortic endothelial cells contain three types of MT genes. namely, MT-1A. MT-1E, and MT-2A; however, the associated protein expression of these MT isoforms has not been identified. In the present study, the expression of MT subisoform proteins in cells treated with cadmium chloride was identified using a high-performance liquid chromatography-inductively coupled plasma-mass spectrometry system. It was revealed that: (1) transcriptional induction of MT-1A by cadmium was markedly more sensitive than that of MT-1E/2A; (2) MT-1A and MT-2A proteins were the predominant MT subisoforms induced by cadmium and (3) there might be differentiation in the functions of MT-1 and MT-2 against cadmium cytotoxicity, although the actual roles of the MT isoforms in the cells were not distinct. This is the first study to show the differential induction of isoforms of MT proteins in vascular endothelial cells by cadmium.