The short neuropeptide F modulates olfactory sensitivity of Bactrocera dorsalis upon starvation
The short neuropeptide F modulates olfactory sensitivity of Bactrocera dorsalis upon starvation
复制标题
短神经肽 F 调节桔小实蝇饥饿时的嗅觉敏感性
DOI：
10.1016/j.jinsphys.2017.03.012
复制
发表时间：
2017-05-01
影响因子：
2.2
通讯作者：
Wang, Jin-Jun
中科院分区：
文献类型：
--
作者：
Jiang, Hong-Bo;Gui, Shun-Hua;Wang, Jin-Jun
昆虫短神经肽F （sNPF）家族已被证明调节多种生理过程，如摄食、食欲嗅觉行为、运动、睡眠稳态和激素释放。在本研究中，我们鉴定了一种重要的农业害虫——桔小实蝇（Bactrocera dorsalis, Hendel）的sNPF （BdsNPF）及其受体（BdsNPFR）。随后，受体cDNA在中国仓鼠卵巢细胞系中功能表达。sNPF肽激活BdsNPFR导致细胞内钙离子增加，在纳摩尔水平上有效浓度达到50%。qPCR结果显示，BdsNPF和BdsNPFR转录本主要在中枢神经系统和触角中检测到，并表现出明显的饥饿诱导表达模式。此外，我们发现，与正常喂食的苍蝇相比，饥饿的苍蝇有增加的天线图反应。然而，当我们通过双链RNA注射降低成人BdsNPF的表达时，这种增强的嗅觉敏感性被逆转。结果表明，sNPF在调节dorsalis在饥饿状态下的嗅觉敏感性中起重要作用。本研究结果将有助于了解背孢霉早期嗅觉加工的调控机制。
The insect short neuropeptide F (sNPF) family has been shown to modulate diverse physiological processes, such as feeding, appetitive olfactory behavior, locomotion, sleep homeostasis and hormone release. In this study, we identified the sNPF (BdsNPF) and its receptor (BdsNPFR) in an important agricultural pest, the oriental fruit fly Bactrocera dorsalis (Hendel). Afterwards, the receptor cDNA was functionally expressed in Chinese hamster ovary cell lines. Activation of BdsNPFR by sNPF peptides caused an increase in intracellular calcium ions, with a 50% effective concentration values at the nanomolar level. As indicated by qPCR, the BdsNPF and BdsNPFR transcripts were mainly detected in the central nervous system and antennae, and they showed significantly starvation-induced expression patterns. Furthermore, we found that the starved flies had an increased electroantennogram response compared to the normally fed flies. However, this enhanced olfactory sensitivity was reversed when we decreased the expression of BdsNPF by double-stranded RNA injection in adults. We concluded that sNPF plays an important role in modulating the olfactory sensitivity of B. dorsalis upon starvation. Our results will facilitate the understanding of the regulation of early olfactory processing in B. dorsalis.