A method for counting acid-fast bacteria.
A method for counting acid-fast bacteria.
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一种抗酸细菌计数方法。
DOI：
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发表时间：
1968
期刊：
影响因子：
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通讯作者：
D. H. McRae
中科院分区：
文献类型：
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作者：
C. Shepard;D. H. McRae
一种可靠、方便、准确的抗酸杆菌（AFB）计数方法是麻风分枝杆菌研究的基础。使用的程序，以前在实验室（~，4）有两个技术上的困难：（a）为了使液滴传播令人满意的显微镜载玻片必须有一个正确的程度均匀的润湿性;有时这一要求是难以satisfy和一个令人满意的程序为一批载玻片有时是不满意的下一批。(b)在干燥过程中，没有组织的悬浮液中的细菌倾向于集中在液滴的外围。因此，开发了一种新方法，并且在下面描述的测试之后，它取代了以前的方法。
A reliable, convenient, and accurate method for counting ac id-fast hacteria ( AFB) is essential for much work with Mycobacterium leprae. The procedure used , previously in the laboratory (~, 4 ) had two technical difficulties: (a) In order for the drops to spread satisfactorily the microscope slides had to have a uniform wetability of the correct degree; sometimes this requirement was difficult to sa tisfy and a satisfactory procedure for one batch of slides was sometimes unsatisfactory for the next batch. (b) There was a tendency for bacteria in suspensions devoid of tissue to concentrate in the periphery of the drop during drying. Consequently a new method was developed, and, after the tes ts described below, it was substituted for the previous method.