Isolation and Genetic Characterization of a Bartonella Strain Closely Related to Bartonella tribocorum and Bartonella elizabethae in Israeli Commensal Rats
Isolation and Genetic Characterization of a Bartonella Strain Closely Related to Bartonella tribocorum and Bartonella elizabethae in Israeli Commensal Rats
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DOI：
10.4269/ajtmh.2009.81.55
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发表时间：
2009-07-01
影响因子：
3.3
通讯作者：
Baneth, Gad
中科院分区：
文献类型：
--
作者：
Harrus, Shimon;Bar-Gal, Gilla Kahila;Baneth, Gad
从从特拉维夫地区捕获的黑鼠（Rattus rattus）采集的血液中培养10株巴尔通体分离株。遗传表征包括5个基因片段的扩增和测序，包括ribC、rpoB、16 S、groEL和gltA以及16 S-23 S基因间间隔区。序列比较显示，所有10个分离株在研究的所有基因中均相同，共分析了3，873 bp。分析的每个部分基因的序列表明与B的高度序列相似性（97-99.8%）。tribocorum或B.伊丽莎白。gltA序列与R.孟加拉国达卡的黑鼠，表明存在广泛的亚洲巴尔通体菌株感染黑鼠（R. rattus）。与B密切相关的巴尔通体基因型的检测。以色列最大的城市中心伊丽莎白值得进一步研究其人畜共患病的潜力和致病特性。
Ten Bartonella isolates were cultured from blood drawn from black rats (Rattus rattus) captured in the Tel Aviv area. Genetic characterization included amplification and sequencing of five gene fragments including the ribC, rpoB, 16S, groEL, and gltA and the 16S-23S intergenic spacer region. Sequence comparisons showed that all 10 isolates were identical in all genes studied comprising a total of 3,873 bp analyzed. The sequences of each of the partial genes analyzed indicated a high sequence similarity (97-99.8%) to B. tribocorum or B. elizabethae. The gltA sequence was 100% homologous to a genotype identified in R. rattus in Dhaka, Bangladesh, suggesting the existence of a widespread Asian Bartonella strain infecting the black rats (R. rattus). The detection of a Bartonella genotype closely related to B. elizabethae in the biggest metropolitan center in Israel warrants further study of its zoonotic potential and pathogenic characteristics.