Identification and morphological classification of horizontal, bipolar, and amacrine cells within the zebrafish retina
Identification and morphological classification of horizontal, bipolar, and amacrine cells within the zebrafish retina
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DOI：
10.1002/cne.20261
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发表时间：
2004-09-27
影响因子：
2.5
通讯作者：
Nelson, R
中科院分区：
文献类型：
--
作者：
Connaughton, VP;Graham, D;Nelson, R
水平，双极，和无长突细胞在斑马鱼视网膜的形态特征，使用DiOlistic技术。在该方法中，将1，1 '-双十八烷基-3，3，3'，3 '-四甲基吲哚羰花青高氯酸盐（DiI）包被的微载体高速喷射到活视网膜切片的表面上，然后DiI在活视网膜切片的表面描绘分离的神经元的轴突、胞体和树突。斑马鱼视网膜胞体直径为5-10 μ m。三个水平的细胞类型（H-A-1，H-A-2，和H-B）进行了鉴定，树突状树直径平均为25-40 μ m。H-A胞体是圆形的。分类为H-A-2的细胞比H-A-1细胞大，并且具有轴突。H-A胞体扁平，无轴突，但从索马伸出短梭形结构。双极细胞分为17种形态类型。树突状树范围从10到70 μ M。B-on型6例，轴突终末仅在内丛状层的ON亚层; B-off型7例，轴突终末或分支仅在内丛状层的OFF亚层。四种类型的双层双极细胞在ON和OFF层均显示出终扣。无长突细胞有7种类型。A（off）细胞（三种类型）在IPL OFF亚层中呈单层和分枝状。树突区域为60-150 μ M。A（上）梨形细胞（3种类型）在ON亚板上分支。树突区域为50-170 μ M。A（弥漫性）细胞在所有IPL层中表达过程。树突区域为15-90 μ M。这些发现对于研究斑马鱼视网膜中的信号处理以及理解视网膜组织突变和扰动导致的功能变化非常重要。
Horizontal, bipolar, and amacrine cells in the zebrafish retina were morphologically characterized using DiOlistic techniques. In this method, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI)-coated microcarriers are shot at high speed onto the surfaces of living retinal slices where the DiI then delineates axons, somata, and dendrites of isolated neurons. Zebrafish retinal somata were 5-10 mum in diameter. Three horizontal cell types (H-A-1, H-A-2, and H-B) were identified; dendritic tree diameters averaged 25-40 mum. H-A somata were round. Cells classified as H-A-2 were larger than H-A-1 cells and possessed an axon. H-A somata were flattened, without an axon, although short fusiform structure(s) projected from the soma. Bipolar cells were separated into 17 morphological types. Dendritic trees ranged from 10 to 70 muM. There were six B-on types with axon boutons only in the ON sublamina of the inner plexiform layer (IPL), and seven B-off types with axon boutons or branches only in the OFF sublamina. Four types of bistratified bipolar cells displayed boutons in both ON and OFF layers. Amacrine cells occurred in seven types. A(off) cells (three types) were monostratified and ramified in the IPL OFF sublamina. Dendritic fields were 60-150 muM. A(on) pyriform cells (three types) branched in the ON sublamina. Dendritic fields were 50-170 muM. A(diffuse) cells articulated processes in all IPL strata. Dendritic fields were 15-90 muM. These findings are important for studies examining signal processing in zebrafish retina and for understanding changes in function resulting from mutations and perturbations of retinal organization.