Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus.
Comparison of β-Propiolactone and Formalin Inactivation on Antigenicity and Immune Response of West Nile Virus.
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DOI：
10.1155/2015/616898
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发表时间：
2015
影响因子：
2.2
通讯作者：
Mahanta J
中科院分区：
文献类型：
--
作者：
Chowdhury P;Topno R;Khan SA;Mahanta J
西尼罗病毒（West Nile Virus，WNV）是黄病毒科黄病毒属的一种致病性虫媒病毒。到目前为止，还没有针对西尼罗河病毒的批准疫苗供人类使用。在本研究中，基于灭活WNV的抗原性和免疫原性潜力，评估了通常用于灭活疫苗制备的两种烷化剂甲醛和β-PL的作用。用福尔马林和β-PL处理使谱系5的WNV分离物失活。通过在BHK-21细胞系和幼年小鼠中重复传代确认灭活。两种处理灭活的病毒均表现出较高的抗原性。小鼠免疫反应显示，福尔马林灭活抗原的血清抗WNV抗体效价略高于β-PL灭活抗原。然而，在中和抗体滴度方面未观察到显著差异。总之，我们可以说甲醛和β-PL灭活过程对WNV的灭活同样有效。但是，随着对细胞介导的免疫应答的研究，需要将它们与其他灭活剂进行沿着比较。
West Nile Virus (WNV) is a pathogenic arbovirus that belongs to genus Flavivirus under family Flaviviridae. Till now there are no approved vaccines against WNV for human use. In this study, the effect of two alkylating agents, formaldehyde and β-PL, generally used for inactivated vaccine preparation, was assessed on the basis of antigenic and immunogenic potential of the inactivated WNV. Lineage 5 WNV isolates were inactivated by both formalin and β-PL treatments. Inactivation was confirmed by repeated passage in BHK-21 cell line and infant mice. Viruses inactivated by both the treatments showed higher antigenicity. Immune response in mice model showed serum anti-WNV antibody titre was moderately higher in formalin inactivated antigen compared to β-PL inactivated antigen. However, no significant differences were observed in neutralization antibody titre. In conclusion, we can state that both formaldehyde and β-PL inactivation processes were found to be equally efficient for inactivation of WNV. However, they need to be compared with other inactivating agents along with study on cell mediated immune response.