Localization of actin and characterization of its isoforms in the hyphae of Neurospora crassa.
Localization of actin and characterization of its isoforms in the hyphae of Neurospora crassa.
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DOI：
10.1111/j.1574-6968.1991.tb04315.x
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发表时间：
1991
影响因子：
2.1
通讯作者：
F. Barja;B. N. Thi;G. Turian
中科院分区：
文献类型：
--
作者：
F. Barja;B. N. Thi;G. Turian
对粗糙脉孢菌野生型菌株St. Lawrence的肌动蛋白进行了纯化、表征和定位。通过DNase I-Sepharose亲和层析分离真菌43 kDa蛋白。当使用抗鸡砂囊的抗肌动蛋白单克隆抗体作为探针时，在免疫印迹上将该蛋白鉴定为肌动蛋白。双向凝胶电泳后，检测到三种肌动蛋白亚型。用荧光素标记的鬼笔环肽对甲醛固定的菌丝进行染色，观察肌动蛋白在菌丝中的分布。F-肌动蛋白主要集中在菌丝顶端，形成一个均匀的帽状结构。顶端肌动蛋白可能参与菌丝形态发生、细胞器运动和极性维持。
The actin of Neurospora crassa wild type Strain St. Lawrence has been purified, characterized and localized. A fungal 43 kDa protein was isolated by affinity chromatography on DNase I-Sepharose. This protein was identified as actin on immunoblots when an anti-actin monoclonal antibody raised against chicken gizzards was used as a probe. After two-dimensional gel electrophoresis three actin isoforms were detected. The distribution of actin in hyphae was examined by FITC-phalloidin staining of formaldehyde fixed hyphae. F-actin was found to be mainly concentrated in the hyphal tips in which it formed a uniform cap. Apical actin could be involved in hyphal morphogenesis, organelle motility and maintenance of polarity.