Measurement of leucine enkephalin in caudate nucleus tissue with fast atom bombardment-collision activated dissociation-linked field scanning mass spectrometry.
Measurement of leucine enkephalin in caudate nucleus tissue with fast atom bombardment-collision activated dissociation-linked field scanning mass spectrometry.
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用快原子轰击碰撞激活解离联场扫描质谱法测量尾状核组织中的亮氨酸脑啡肽。
DOI：
10.1002/bms.1200100707
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Kai,M
中科院分区：
文献类型：
--
作者：
Desiderio,DM;Katakuse,I;Kai,M
使用保留绝对分子特异性的质谱分析方法测量犬尾状核组织提取物中阿片类五肽亮氨酸脑啡肽的内源量。快速原子轰击质谱产生亮氨酸脑啡肽的质子化分子离子，然后碰撞激活解离产生氨基酸序列决定离子。通过链接场 (B/E) 扫描分析这些氨基酸序列决定离子。选择一种氨基酸序列决定离子来测量内源性亮氨酸脑啡肽。这种新颖的测量模式为犬尾状核生物组织提取物中亮氨酸脑啡肽的内源量（451 pmol g−1组织）的定量提供了最佳的分子特异性。
The endogenous amount of the opioid pentapeptide leucine enkephalin was measured in a canine caudate nucleus tissue extract using mass spectral analytical methods which retain absolute molecular specificity. Fast atom bombardment mass spectrometry generation of the protonated molecular ion of leucine enkephalin followed by collision activated dissociation produced amino acid sequence‐determining ions. These amino acid sequence‐determining ions were analyzed by a linked field (B/E) scan. One amino acid sequence‐determining ion was selected to measure endogenous leucine enkephalin. This novel measurement mode offers optimal molecular specificity for quantification of an endogenous amount (451 pmol g−1tissue) of leucine enkephalin in a biologic tissue extract of canine caudate nucleus.