Basic research on aw-AC/PLGA composite scaffolds for bone tissue engineering
Basic research on aw-AC/PLGA composite scaffolds for bone tissue engineering
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DOI：
10.1007/s10856-007-0162-x
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发表时间：
2008-03-01
影响因子：
3.7
通讯作者：
Nagayama, Masaru
中科院分区：
文献类型：
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作者：
Minamiguchi, Shiho;Takechi, Masaaki;Nagayama, Masaru
近年来，开发有效的骨组织工程支架材料变得十分重要。因此，我们制备了由可生物降解的聚(D，L-丙交酯-乙醇酸)(PLGA)(75/25)和抗洗脱型AC(Aw-AC)颗粒组成的新型三维支架。本研究的目的是评价这种新型支架的基本性能和生物相容性。用扫描电子显微镜(SEM)观察了所获得的支架，并测量了孔隙率、收缩和双向抗压强度。结果表明，含Aw-AC复合支架(Aw-AC/PL)的PLGA比PLGA支架(PL)具有更高的强度和稳定性。与PL相比，Aw-AC/PL在孵化过程中的质量减少量也有所减少。分别于接种后5h、1周、2周计数MC3T3-E1细胞数。结果表明，Aw-AC/PL在附着和增殖方面表现出优于PL的性能。组织学上，Aw-AC/PL对软组织有良好的反应。因此，Aw-AC/PL比PL具有更好的生物相容性。综上所述，Aw-AC/PL在骨组织工程中比PL更适用于细胞移植。
Recently, it has become important to develop effective material to be used as scaffolds for bone tissue engineering. Therefore, we fabricated new three-dimensional (3D) scaffolds consisting of biodegradable poly(D,L-lactide-co-glycolic acid)(PLGA)(75/25) with anti-washout type AC (aw-AC) particles. The aim of this study was to evaluate this new scaffold concerning its basic properties and biocompatibility. The obtained scaffolds were observed with scanning electron microscopy (SEM), and measured for porosity, shrinkage and biaxial compressive strengths. It was shown that PLGA with aw-AC composite scaffolds (aw-AC/PL) showed a greater strength and stability than PLGA scaffolds (PL). Also, the mass reduction of aw-AC/PL during incubation decreased compared to that of PL. The number of MC3T3-E1 cell in PL and aw-AC/PL was counted at 5 h, 1 week, and 2 weeks after cell seeding. As a result, aw-AC/PL exhibited a superior performance in terms of attachment and proliferation compared to PL. Histologically, aw-AC/PL showed an excellent response toward soft tissues. Therefore, it was shown that aw-AC/PL was more biocompatible than PL. In conclusion, it was strongly suggested that aw-AC/PL was more useful for cell transplantation than PL in bone tissue engineering.