Amino acid replacements and wavelength absorption of visual pigments in vertebrates.
Amino acid replacements and wavelength absorption of visual pigments in vertebrates.
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DOI：
10.1093/oxfordjournals.molbev.a040190
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发表时间：
1995
影响因子：
10.7
通讯作者：
Shozo Yokoyama
中科院分区：
文献类型：
--
作者：
Shozo Yokoyama
光转导中一个重要的未回答的问题是视色素（VP）如何调节其最大吸收波长（λ max）。通过对已知λ max值的28个视蛋白构建进化树，我们可以确定不同VP的λ max移位的时间和方向。总共有55个氨基酸的变化与λ最大位移的方向相关，并且可能在确定VP的λ最大值中很重要。其中，三个氨基酸变化已被证明是将绿色敏感VP修饰为红色敏感VP的原因。目前的进化分析打开了一个新的方向，在理解的机制，由VP的波长吸收的调节，更一般地说，在研究分子机制参与适应性进化。
An important unanswered question in phototransduction is how visual pigments (VPs) regulate their wavelength of maximal absorption (lambda max). By constructing the evolutionary tree for 28 opsins with known lambda max values, we can identify the times and directions of lambda max shift of different VPs. A total of 55 amino acid changes are shown to correlate with the directions of lambda max shift and might have been important in determining lambda max of a VP. Among these, three amino acid changes are already proven to be responsible in modifying the green-sensitive VP to the red-sensitive VP. The present evolutionary analysis opens a new direction in understanding the mechanism for the regulation of wavelength absorption by a VP and, more generally, in studying molecular mechanism involved in adaptive evolution.