Involvement of Rho-type GTPase in control of cell size in Saccharomyces cerevisiae
Involvement of Rho-type GTPase in control of cell size in Saccharomyces cerevisiae
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DOI：
10.1111/j.1567-1364.2007.00213.x
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发表时间：
2007-06-01
影响因子：
3.2
通讯作者：
Ohya, Yoshikazu
中科院分区：
文献类型：
--
作者：
Kikuchi, Yo;Mizuuchi, Eri;Ohya, Yoshikazu
维持特定的细胞大小，这对许多生物体很重要，是通过协调细胞生长和细胞分裂来实现的。在芽殖酵母Saccharomycescerevisiae中，提出了两个细胞大小检查点的存在：在第一个检查点处，在G（1）/S转变的芽殖之前监测细胞大小;在第二个检查点处，在G（2）/M转变之前监测小芽中发生的肌动蛋白解聚。形态学分析表明，小的GTdR Rho 1 p在G（1）/S和G（2）/M边界参与细胞大小的控制。一组rho 1突变体（rho 1A）过早进入有丝分裂，导致异常小的细胞诞生。在另一组rho 1突变体（rho 1B）中，母细胞在出芽前未能达到适当的大小，G（1）细胞周期蛋白Cln 2 p的表达在细胞周期的早期阶段开始。Rho 1 p效应蛋白缺陷突变体的分析表明，Skn 7 p，Fks 1 p和Mpk 1 p参与细胞大小的控制。因此，Rho 1 p及其下游调控途径参与了S.啤酒。
Maintaining specific cell size, which is important for many organisms, is achieved by coordinating cell growth and cell division. In the budding yeast Saccharomyces cerevisiae, the existence of two cell-size checkpoints is proposed: at the first checkpoint, cell size is monitored before budding at the G(1)/S transition, and at the second checkpoint, actin depolymerization occurring in the small bud is monitored before the G(2)/M transition. Morphological analyses have revealed that the small GTPase Rho1p participates in cell-size control at both the G(1)/S and the G(2)/M boundaries. One group of rho1 mutants (rho1A) underwent premature entry into mitosis, leading to the birth of abnormally small cells. In another group of rho1 mutants (rho1B), the mother cells failed to reach an appropriate size before budding, and expression of the G(1) cyclin Cln2p began at an earlier phase of the cell cycle. Analyses of mutants defective in Rho1p effector proteins indicate that Skn7p, Fks1p and Mpk1p are involved in cell-size control. Thus, Rho1p and its downstream regulatory pathways are involved in controlling cell size in S. cerevisiae.